Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Ag+ | - |
Pseudomonas aeruginosa | |
CuCl | - |
Pseudomonas aeruginosa | |
CuSO4 | - |
Pseudomonas aeruginosa | |
o-phenanthroline | - |
Pseudomonas aeruginosa |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.002 | - |
catechol | metapyrocatechase 2 | Pseudomonas aeruginosa | |
0.0041 | - |
catechol | metapyrocatechase 1 | Pseudomonas aeruginosa | |
0.008 | - |
4-methylcatechol | metapyrocatechase 2 | Pseudomonas aeruginosa | |
0.01 | - |
3-methylcatechol | metapyrocatechase 2 | Pseudomonas aeruginosa | |
0.02 | - |
4-methylcatechol | metapyrocatechase 1 | Pseudomonas aeruginosa | |
0.06 | - |
3-methylcatechol | metapyrocatechase 1 | Pseudomonas aeruginosa |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Fe2+ | FeCl2 and FeSO4 activate metapyrocatechase 2, no effect on metapyrocatechase 1 | Pseudomonas aeruginosa |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
33500 | - |
4 * 33500 metapyrocatechase 1, SDS-PAGE | Pseudomonas aeruginosa |
36000 | - |
4 * 36000, metapyrocatechase 2, SDS-PAGE | Pseudomonas aeruginosa |
132000 | 134000 | gel filtration, sedimentation equilibrium analysis, amino acid analysis, metapyrocatechase 2 | Pseudomonas aeruginosa |
143000 | 144600 | gel filtration, sedimentation equilibrium analysis, amino acid analysis | Pseudomonas aeruginosa |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
catechol + O2 | Pseudomonas aeruginosa | metapyrocatechase 1 takes part in oxidation of aromatic compounds, producing catechol as an intermediate, metapyrocatechase 2 is involved in oxidation of methyl-substituted aromatic substrates | 2-hydroxymuconate semialdehyde | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas aeruginosa | - |
- |
- |
Purification (Comment) | Organism |
---|---|
metapyrocatechase 1 and 2 | Pseudomonas aeruginosa |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
35 | - |
metapyrocatechase 2 | Pseudomonas aeruginosa |
90 | - |
metapyrocatechase 1 | Pseudomonas aeruginosa |
Storage Stability | Organism |
---|---|
-20°C, in lyophilized state for several months, metapyrocatechase 1 and 2 | Pseudomonas aeruginosa |
4°C, in acetone buffer under nitrogen for several weeks, metapyrocatechase 1 and 2 | Pseudomonas aeruginosa |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
3,4-dihydroxybenzoate + O2 | weak | Pseudomonas aeruginosa | ? | - |
? | |
3-chlorocatechol + O2 | weak | Pseudomonas aeruginosa | 2-chloro-2-hydroxy-6-oxohexa-2,4-dienoate | - |
? | |
3-methylcatechol + O2 | weak | Pseudomonas aeruginosa | 2-hydroxy-6-oxohepta-2,4-dienoate | - |
? | |
4-methylcatechol + O2 | - |
Pseudomonas aeruginosa | 2-hydroxy-3-methyl-6-oxohexa-2,4-dienoate | - |
? | |
catechol + O2 | - |
Pseudomonas aeruginosa | 2-hydroxymuconate semialdehyde | - |
? | |
catechol + O2 | metapyrocatechase 1 takes part in oxidation of aromatic compounds, producing catechol as an intermediate, metapyrocatechase 2 is involved in oxidation of methyl-substituted aromatic substrates | Pseudomonas aeruginosa | 2-hydroxymuconate semialdehyde | - |
? |
Subunits | Comment | Organism |
---|---|---|
tetramer | 4 * 33500 metapyrocatechase 1, SDS-PAGE | Pseudomonas aeruginosa |
tetramer | 4 * 36000, metapyrocatechase 2, SDS-PAGE | Pseudomonas aeruginosa |