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Literature summary for 1.13.11.2 extracted from

  • Jeong, J.J.; Kim, J.H.; Kim, C.K.; Hwang, I.; Lee, K.
    3- and 4-alkylphenol degradation pathway in Pseudomonas sp. strain KL28: genetic organization of the lap gene cluster and substrate specificities of phenol hydroxylase and catechol 2,3-dioxygenase (2003), Microbiology, 149, 3265-3277.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Pseudomonas putida G7.C-1 and Escherichia coli DH5alpha Pseudomonas sp.

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4-alkylcatechol + O2 Pseudomonas sp. initial step of long-chain alkylphenol cleavage pathway ?
-
?
4-alkylcatechol + O2 Pseudomonas sp. KL28 initial step of long-chain alkylphenol cleavage pathway ?
-
?
catechol + O2 Pseudomonas sp. involved in metabolism of aromatic compounds 2-hydroxymuconate semialdehyde
-
?
catechol + O2 Pseudomonas sp. KL28 involved in metabolism of aromatic compounds 2-hydroxymuconate semialdehyde
-
?

Organism

Organism UniProt Comment Textmining
Pseudomonas sp. Q7WYF5
-
-
Pseudomonas sp. KL28 Q7WYF5
-
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
10
-
with 2,3-dihydroxybiphenyl, pH 7.5, 28°C Pseudomonas sp.
20
-
with catechol, pH 7.5, 28°C Pseudomonas sp.
68
-
with 3-methylcatechol, pH 7.5, 28°C Pseudomonas sp.
320
-
with 4-ethylcatechol, pH 7.5, 28°C Pseudomonas sp.
593
-
with 4-methylcatechol, pH 7.5, 28°C Pseudomonas sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2,3-dihydroxybiphenyl + O2 poor substrate Pseudomonas sp. ?
-
?
2,3-dihydroxybiphenyl + O2 poor substrate Pseudomonas sp. KL28 ?
-
?
3-methylcatechol + O2 poor substrate Pseudomonas sp. 2-hydroxy-3-methylmuconate semialdehyde
-
?
4-alkylcatechol + O2 initial step of long-chain alkylphenol cleavage pathway Pseudomonas sp. ?
-
?
4-alkylcatechol + O2 alkyl chain length C1-C5 is accepted as substrate Pseudomonas sp. ?
-
?
4-alkylcatechol + O2 initial step of long-chain alkylphenol cleavage pathway Pseudomonas sp. KL28 ?
-
?
4-alkylcatechol + O2 alkyl chain length C1-C5 is accepted as substrate Pseudomonas sp. KL28 ?
-
?
4-ethylcatechol + O2 effective substrate Pseudomonas sp. 4-ethyl-2-hydroxymuconate semialdehyde
-
?
4-methylcatechol + O2 very effective substrate Pseudomonas sp. 2-hydroxy-4-methylmuconate semialdehyde
-
?
catechol + O2 poor substrate Pseudomonas sp. 2-hydroxymuconate semialdehyde
-
?
catechol + O2 involved in metabolism of aromatic compounds Pseudomonas sp. 2-hydroxymuconate semialdehyde
-
?
catechol + O2 poor substrate Pseudomonas sp. KL28 2-hydroxymuconate semialdehyde
-
?
catechol + O2 involved in metabolism of aromatic compounds Pseudomonas sp. KL28 2-hydroxymuconate semialdehyde
-
?