Cloned (Comment) | Organism |
---|---|
expressed in Pseudomonas putida G7.C-1 and Escherichia coli DH5alpha | Pseudomonas sp. |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-alkylcatechol + O2 | Pseudomonas sp. | initial step of long-chain alkylphenol cleavage pathway | ? | - |
? | |
4-alkylcatechol + O2 | Pseudomonas sp. KL28 | initial step of long-chain alkylphenol cleavage pathway | ? | - |
? | |
catechol + O2 | Pseudomonas sp. | involved in metabolism of aromatic compounds | 2-hydroxymuconate semialdehyde | - |
? | |
catechol + O2 | Pseudomonas sp. KL28 | involved in metabolism of aromatic compounds | 2-hydroxymuconate semialdehyde | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas sp. | Q7WYF5 | - |
- |
Pseudomonas sp. KL28 | Q7WYF5 | - |
- |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
10 | - |
with 2,3-dihydroxybiphenyl, pH 7.5, 28°C | Pseudomonas sp. |
20 | - |
with catechol, pH 7.5, 28°C | Pseudomonas sp. |
68 | - |
with 3-methylcatechol, pH 7.5, 28°C | Pseudomonas sp. |
320 | - |
with 4-ethylcatechol, pH 7.5, 28°C | Pseudomonas sp. |
593 | - |
with 4-methylcatechol, pH 7.5, 28°C | Pseudomonas sp. |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2,3-dihydroxybiphenyl + O2 | poor substrate | Pseudomonas sp. | ? | - |
? | |
2,3-dihydroxybiphenyl + O2 | poor substrate | Pseudomonas sp. KL28 | ? | - |
? | |
3-methylcatechol + O2 | poor substrate | Pseudomonas sp. | 2-hydroxy-3-methylmuconate semialdehyde | - |
? | |
4-alkylcatechol + O2 | initial step of long-chain alkylphenol cleavage pathway | Pseudomonas sp. | ? | - |
? | |
4-alkylcatechol + O2 | alkyl chain length C1-C5 is accepted as substrate | Pseudomonas sp. | ? | - |
? | |
4-alkylcatechol + O2 | initial step of long-chain alkylphenol cleavage pathway | Pseudomonas sp. KL28 | ? | - |
? | |
4-alkylcatechol + O2 | alkyl chain length C1-C5 is accepted as substrate | Pseudomonas sp. KL28 | ? | - |
? | |
4-ethylcatechol + O2 | effective substrate | Pseudomonas sp. | 4-ethyl-2-hydroxymuconate semialdehyde | - |
? | |
4-methylcatechol + O2 | very effective substrate | Pseudomonas sp. | 2-hydroxy-4-methylmuconate semialdehyde | - |
? | |
catechol + O2 | poor substrate | Pseudomonas sp. | 2-hydroxymuconate semialdehyde | - |
? | |
catechol + O2 | involved in metabolism of aromatic compounds | Pseudomonas sp. | 2-hydroxymuconate semialdehyde | - |
? | |
catechol + O2 | poor substrate | Pseudomonas sp. KL28 | 2-hydroxymuconate semialdehyde | - |
? | |
catechol + O2 | involved in metabolism of aromatic compounds | Pseudomonas sp. KL28 | 2-hydroxymuconate semialdehyde | - |
? |