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Literature summary for 1.13.11.2 extracted from

  • Sugimoto, K.; Matsufuzi, K.; Ohnuma, H.; Senda, M.; Fukuda, M.; Senda, T.
    Crystallization and preliminary crystallographic analysis of the catechol 2,3-dioxygenase PheB from Bacillus stearothermophilus BR219 (2006), Acta Crystallogr. Sect. F, F62, 125-127.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.13.11.2

Activating Compound

Activating Compound Comment Organism Structure
ascorbate incubation of the purified enzyme (PheB) with Fe2+ and ascorbate increases its activity by approximately sevenfold Geobacillus stearothermophilus

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli (JM109) Geobacillus stearothermophilus

Crystallization (Commentary)

Crystallization (Comment) Organism
Crystallization of the iron incubated PheB enzyme. Hanging-drop vapour diffusion method with best crystals after 7 d at 285 K using 0.2 M Li2SO4, 15% PEG 4000 and 0.1 M HEPES pH 7.0 in the reservoir and protein solution with a concentration of 40 mg/ml. The crystal diffracts to 2.3 A resolution Geobacillus stearothermophilus

Metals/Ions

Metals/Ions Comment Organism Structure
Fe2+ incubation of the purified enzyme (PheB) with Fe2+ and ascorbate increases its activity by approximately sevenfold Geobacillus stearothermophilus

Organism

Organism UniProt Comment Textmining
Geobacillus stearothermophilus
-
-
-
Geobacillus stearothermophilus BR219
-
-
-

Purification (Commentary)

Purification (Comment) Organism
hydrophobic interaction column pre-equilibrated with 30% (w/v) ammonium sulfate and 0.1 M potassium phosphate buffer pH 7.5, protein eluation with ammonium sulfate gradient [30-0% (w/v)]. DEAE column pre-equilibrated with 50 mM potassium phosphate buffer pH 7.5, protein eluation with NaCl gradient (80-400 mM) Geobacillus stearothermophilus

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
 0.4 U/mg, purified enzyme Geobacillus stearothermophilus

Subunits

Subunits Comment Organism
More The space group of the crystal is found to be P212121, with unit-cell parameters a = 65.5, b = 119.2, c = 158.7 A, assuming the presence of one tetramer in the asymmetric unit Geobacillus stearothermophilus

Synonyms

Synonyms Comment Organism
ECDO
-
 Geobacillus stearothermophilus
Extradiol-cleaving catecholic dioxygenase
-
 Geobacillus stearothermophilus
PheB one of the XylE-group enzyms Geobacillus stearothermophilus
XylE one extradiol-cleaving catecholic dioxygenases group Geobacillus stearothermophilus