Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Saccharolobus solfataricus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0016 | - |
catechol | pH 7.5, 25°C | Saccharolobus solfataricus | |
0.0017 | - |
3-methylcatechol | pH 7.5,25 °C | Saccharolobus solfataricus | |
0.003 | - |
4-methylcatechol | pH 7.5, 25°C | Saccharolobus solfataricus | |
0.0036 | - |
3-methylcatechol | pH 7.5, 60°C | Saccharolobus solfataricus | |
0.0059 | - |
catechol | pH 7.5, 60°C | Saccharolobus solfataricus | |
0.012 | - |
4-methylcatechol | pH 7.5, 60°C | Saccharolobus solfataricus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Fe2+ | the enzyme contains about 2 mol of Fe2+ per mole of protein | Saccharolobus solfataricus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
catechol + O2 | Saccharolobus solfataricus | the enzyme is constitutively transcribed | 2-hydroxymuconate-6-semialdehyde | - |
? | |
catechol + O2 | Saccharolobus solfataricus P2 | the enzyme is constitutively transcribed | 2-hydroxymuconate-6-semialdehyde | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Saccharolobus solfataricus | V5W6K5 | - |
- |
Saccharolobus solfataricus P2 | V5W6K5 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Saccharolobus solfataricus |
Storage Stability | Organism |
---|---|
4°C, the enzyme retains complete activity for more than 350 min when kept under an N2 atmosphere, whereas it loses about 80% of its activity when stored for the same time in the absence of nitrogen | Saccharolobus solfataricus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
3-methylcatechol + O2 | - |
Saccharolobus solfataricus | ? | - |
? | |
3-methylcatechol + O2 | - |
Saccharolobus solfataricus P2 | ? | - |
? | |
4-methylcatechol + O2 | - |
Saccharolobus solfataricus | ? | - |
? | |
4-methylcatechol + O2 | - |
Saccharolobus solfataricus P2 | ? | - |
? | |
catechol + O2 | - |
Saccharolobus solfataricus | 2-hydroxymuconate-6-semialdehyde | - |
? | |
catechol + O2 | the enzyme is constitutively transcribed | Saccharolobus solfataricus | 2-hydroxymuconate-6-semialdehyde | - |
? | |
catechol + O2 | - |
Saccharolobus solfataricus P2 | 2-hydroxymuconate-6-semialdehyde | - |
? | |
catechol + O2 | the enzyme is constitutively transcribed | Saccharolobus solfataricus P2 | 2-hydroxymuconate-6-semialdehyde | - |
? |
Synonyms | Comment | Organism |
---|---|---|
SSO1223 | locus name | Saccharolobus solfataricus |
SsoC2,3O | - |
Saccharolobus solfataricus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
70 | 80 | - |
Saccharolobus solfataricus |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | 80 | 50°C: about 50% of maximal activity, 70-80°C: maximal activity | Saccharolobus solfataricus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | - |
half-life: 230-240 min | Saccharolobus solfataricus |
70 | - |
half-life: 100-110 min | Saccharolobus solfataricus |
80 | - |
half-life: about 20 min | Saccharolobus solfataricus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.58 | - |
3-methylcatechol | pH 7.5,25 °C | Saccharolobus solfataricus | |
0.76 | - |
4-methylcatechol | pH 7.5, 25°C | Saccharolobus solfataricus | |
1.3 | - |
catechol | pH 7.5, 25°C | Saccharolobus solfataricus | |
4.7 | - |
4-methylcatechol | pH 7.5, 60°C | Saccharolobus solfataricus | |
5.2 | - |
3-methylcatechol | pH 7.5, 60°C | Saccharolobus solfataricus | |
7.8 | - |
catechol | pH 7.5, 60°C | Saccharolobus solfataricus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
assay at | Saccharolobus solfataricus |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
250 | - |
4-methylcatechol | pH 7.5, 25°C | Saccharolobus solfataricus | |
340 | - |
3-methylcatechol | pH 7.5,25 °C | Saccharolobus solfataricus | |
400 | - |
4-methylcatechol | pH 7.5, 60°C | Saccharolobus solfataricus | |
810 | - |
catechol | pH 7.5, 25°C | Saccharolobus solfataricus | |
1300 | - |
catechol | pH 7.5, 60°C | Saccharolobus solfataricus | |
1400 | - |
3-methylcatechol | pH 7.5, 60°C | Saccharolobus solfataricus |