Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) cells | Planococcus sp. S5 |
Protein Variants | Comment | Organism |
---|---|---|
M65T | the mutant show increased activity compared to the wild type enzyme | Planococcus sp. S5 |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
catechol + O2 | Planococcus sp. S5 | - |
2-hydroxymuconate-6-semialdehyde | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Planococcus sp. S5 | E7DDG2 | - |
- |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.00125 | - |
wild type enzyme, with 3-methylcatechol as substrate, at pH 7.0 and 35°C | Planococcus sp. S5 |
0.00155 | - |
mutant enzyme M65T, with 3-methylcatechol as substrate, at pH 7.0 and 35°C | Planococcus sp. S5 |
0.00938 | - |
wild type enzyme, with 4-chlorocatechol as substrate, at pH 7.0 and 35°C | Planococcus sp. S5 |
0.02065 | - |
wild type enzyme, with catechol as substrate, at pH 7.0 and 35°C | Planococcus sp. S5 |
0.0214 | - |
wild type enzyme, with 4-methylcatechol as substrate, at pH 7.0 and 35°C | Planococcus sp. S5 |
0.02245 | - |
mutant enzyme M65T, with catechol as substrate, at pH 7.0 and 35°C | Planococcus sp. S5 |
0.0312 | - |
mutant enzyme M65T, with 4-methylcatechol as substrate, at pH 7.0 and 35°C | Planococcus sp. S5 |
0.04843 | - |
mutant enzyme M65T, with 4-chlorocatechol as substrate, at pH 7.0 and 35°C | Planococcus sp. S5 |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
3-methylcatechol + O2 | 6.12% activity compared to catechol | Planococcus sp. S5 | 2-hydroxy-6-oxohepta-2,4-dienoate | - |
? | |
4-chlorocatechol + O2 | 44.88% activity compared to catechol | Planococcus sp. S5 | 4-chloro-2-hydroxymuconate semialdehyde | - |
? | |
4-methylcatechol + O2 | 101.91% activity compared to catechol | Planococcus sp. S5 | 2-hydroxy-3-methyl-6-oxohexa-2,4-dienoate | - |
? | |
catechol + O2 | - |
Planococcus sp. S5 | 2-hydroxymuconate-6-semialdehyde | - |
? | |
catechol + O2 | 100% activity | Planococcus sp. S5 | 2-hydroxymuconate-6-semialdehyde | - |
? |
Synonyms | Comment | Organism |
---|---|---|
C23o | - |
Planococcus sp. S5 |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
35 | - |
- |
Planococcus sp. S5 |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
- |
Planococcus sp. S5 |