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Literature summary for 1.13.11.2 extracted from

  • Muthu, M.; Ophir, Y.; Macdonald, L.J.; Vaidya, A.; Lloyd-Jones, G.
    Versatile catechol dioxygenases in Sphingobium scionense WP01T (2018), Antonie van Leeuwenhoek, 111, 2293-2301 .
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli BL21 (DE3) Sphingobium scionense

Organism

Organism UniProt Comment Textmining
Sphingobium scionense A0A346DAQ3
-
-
Sphingobium scionense DSM 19371 A0A346DAQ3
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2,3-dihydroxybiphenyl + O2 9.47% compared to the activity with catechol Sphingobium scionense ?
-
?
2,3-dihydroxybiphenyl + O2 9.47% compared to the activity with catechol Sphingobium scionense DSM 19371 ?
-
?
3-methylcatechol + O2 211.7% compared to the activity with catechol Sphingobium scionense 2-hydroxy-3-methoxymuconate semialdehyde
-
?
4,5-dichlorocatechol + O2 4.6% compared to the activity with catechol Sphingobium scionense ?
-
?
4,5-dichlorocatechol + O2 4.6% compared to the activity with catechol Sphingobium scionense DSM 19371 ?
-
?
4-chlorocatechol + O2 17.4% compared to the activity with catechol Sphingobium scionense 4-chloro-2-hydroxymuconate semialdehyde
-
?
4-chlorocatechol + O2 17.4% compared to the activity with catechol Sphingobium scionense DSM 19371 4-chloro-2-hydroxymuconate semialdehyde
-
?
4-methylcatechol + O2 111.4% compared to the activity with catechol Sphingobium scionense 2-hydroxy-4-methylmuconate semialdehyde
-
?
4-methylcatechol + O2 111.4% compared to the activity with catechol Sphingobium scionense DSM 19371 2-hydroxy-4-methylmuconate semialdehyde
-
?
catechol + O2
-
Sphingobium scionense 2-hydroxymuconate-6-semialdehyde
-
?
catechol + O2
-
Sphingobium scionense DSM 19371 2-hydroxymuconate-6-semialdehyde
-
?

Synonyms

Synonyms Comment Organism
catechol 2,3-dioxygenase
-
Sphingobium scionense
XylE
-
Sphingobium scionense

Expression

Organism Comment Expression
Sphingobium scionense induced by naphthalene, biphenyl and pentachlorophenol up
Sphingobium scionense when used as sole carbon sources benzoate and meta-toluate lead to elevated expression. The induction of xylE is the highest (at 93.5times higher than succinate grown cells) when grown on benzoate or meta-toluate up

General Information

General Information Comment Organism
physiological function the enzyme (XylE) is recruited in pathways that are involved in the degradation of chlorinated aromatic compounds such as pentachlorophenol. It is involved in pathways that generate catechol as a degradation pathway intermediate. XylE will also play a role in degradation pathways that produce alkylcatechols Sphingobium scionense