Literature summary for 1.13.11.2 extracted from

Liu, Z.; Zhang, Y.; Bian, C.; Xia, T.; Gao, Y.; Zhang, X.; Wang, H.; Ma, H.; Hu, Y.; Wang, X.
Highly sensitive microbial biosensor based on recombinant Escherichia coli overexpressing catechol 2,3-dioxygenase for reliable detection of catechol (2019), Biosens. Bioelectron., 126, 51-58 .

Search for more literature for 1.13.11.2

Application

Application	Comment	Organism
analysis	a highly sensitive microbial biosensor is constructed using the recombinant Escherichia coli BL21-C23O cell as bio-recognition element for catechol detection. Based on the synergistic effect of catechol 2,3-dioxygenase and nanoporous gold, the resulting microbial biosensor exhibits good performance for catechol detection with high sensitivity, strong anti-interference and good stability	Sphingobium yanoikuyae

Cloned(Commentary)

Cloned (Comment)	Organism
overexpression in Escherichia coli BL21	Sphingobium yanoikuyae

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.00252	-	catechol	recombinant Escherichia coli BL21-C23O cell, pH and temperature not specified in the publication	Sphingobium yanoikuyae
0.00341	-	catechol	crude enzyme, pH and temperature not specified in the publication	Sphingobium yanoikuyae

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
catechol + O2	Sphingobium yanoikuyae	-	2-hydroxymuconate-6-semialdehyde	-	?
catechol + O2	Sphingobium yanoikuyae XLDN2-5	-	2-hydroxymuconate-6-semialdehyde	-	?

Organism

Organism	UniProt	Comment	Textmining
Sphingobium yanoikuyae	-	-	-
Sphingobium yanoikuyae XLDN2-5	-	-	-

Storage Stability

Storage Stability	Organism
22°C, 9 days, crude enzyme is completely inactivated	Sphingobium yanoikuyae
22°C, 9 days, the catalytic activity of the crude enzyme retains 40% of its original enzymatic activity	Sphingobium yanoikuyae
4°C, 9 days, the catalytic activity of crude enzyme is reduced to approximately 50% of its original enzymatic activity	Sphingobium yanoikuyae
4°C, 9 days, the catalytic activity of the recombinant Escherichia coli BL21-C23O cells retain 90% of its original enzymatic activity	Sphingobium yanoikuyae

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
catechol + O2	-	Sphingobium yanoikuyae	2-hydroxymuconate-6-semialdehyde	-	?
catechol + O2	compared to the enzyme from Sphingobium yanoikuyae XLDN2-5, the recombinant Escherichia coli BL21 overexpressed C23O exhibits higher catalytic activity towards catechol. The whole cells provided a better environment for C23O to maintain its catalytic activity and stability compared with crude enzyme	Sphingobium yanoikuyae	2-hydroxymuconate-6-semialdehyde	-	?
catechol + O2	-	Sphingobium yanoikuyae XLDN2-5	2-hydroxymuconate-6-semialdehyde	-	?
catechol + O2	compared to the enzyme from Sphingobium yanoikuyae XLDN2-5, the recombinant Escherichia coli BL21 overexpressed C23O exhibits higher catalytic activity towards catechol. The whole cells provided a better environment for C23O to maintain its catalytic activity and stability compared with crude enzyme	Sphingobium yanoikuyae XLDN2-5	2-hydroxymuconate-6-semialdehyde	-	?

Synonyms

Synonyms	Comment	Organism
C23o	-	Sphingobium yanoikuyae

Temperature Stability [°C]

Temperature Stability Minimum [°C]	Temperature Stability Maximum [°C]	Comment	Organism
22	-	9 days, crude enzyme is completely inactivated	Sphingobium yanoikuyae

General Information

General Information	Comment	Organism
physiological function	key enzyme in the biodegradation of aromatic compounds	Sphingobium yanoikuyae

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Release 2023.1 (January 2023)