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Literature summary for 1.13.11.20 extracted from

  • Kumar, V.; Maresca, B.; Sacco, M.; Goewert, R.; Kobayashi, G.S.; Medoff, G.
    Purification and characterization of a cysteine dioxygenase from the yeast phase of Histoplasma capsulatum (1983), Biochemistry, 22, 762-768.
    View publication on PubMed
Search for more literature for 1.13.11.20

Activating Compound

Activating Compound Comment Organism Structure
NADH stimulates Histoplasma capsulatum

General Stability

General Stability Organism
pronase destroys activity Histoplasma capsulatum

Inhibitors

Inhibitors Comment Organism Structure
EDTA totally inhibits at very low concentrations Histoplasma capsulatum
EGTA totally inhibits at very low concentrations Histoplasma capsulatum
o-phenanthroline totally inhibits at very low concentrations Histoplasma capsulatum

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.02
-
 L-cysteine
-
 Histoplasma capsulatum
16.6
-
 beta-mercaptoethanol
-
 Histoplasma capsulatum

Localization

Localization Comment Organism GeneOntology No. Textmining
cytosol
-
 Histoplasma capsulatum 5829
-

Metals/Ions

Metals/Ions Comment Organism Structure
Fe stimulation, restores activity after inhibition with EDTA Histoplasma capsulatum

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
10500
-
 gel filtration, SDS-PAGE, the estimate of molecular weight may be inaccurate because it is based on use of the iodinated protein and the assumption that iodination does not affect molecular weight Histoplasma capsulatum

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
L-cysteine + O2 Histoplasma capsulatum probable role in the mycelial to yeast phase transition 3-sulfino-L-alanine
-
 ?

Organism

Organism UniProt Comment Textmining
Histoplasma capsulatum
-
-
-

Purification (Commentary)

Purification (Comment) Organism
using filtration, centrifugation, column chromatography on DEAE-cellulose, Sephadex G-50 and cysteine-Sepharose Histoplasma capsulatum

Source Tissue

Source Tissue Comment Organism Textmining
additional information no activity in mycelium Histoplasma capsulatum
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
 6.31 atoms of oxygen/1000000 * min * mg protein Histoplasma capsulatum

Storage Stability

Storage Stability Organism
-70°C, crude enzyme, stable for up to 4 weeks Histoplasma capsulatum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
beta-mercaptoethanol + O2 slight activity Histoplasma capsulatum 2-hydroxyethanesulfinate
-
 ?
L-cysteine + O2 highly specific for L-cysteine Histoplasma capsulatum 3-sulfino-L-alanine
-
 ?
L-cysteine + O2 probable role in the mycelial to yeast phase transition Histoplasma capsulatum 3-sulfino-L-alanine
-
 ?
additional information D-cysteine, cystine, taurine, cystamine, cysteinesulfinic acid, glutathione, cysteic acid, S-methylcysteine and pyruvic acid do not serve as substrates Histoplasma capsulatum ?
-
 ?

Subunits

Subunits Comment Organism
monomer 1 * 10500, SDS-PAGE Histoplasma capsulatum

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 activity at 37°C 2-fold higher than at 25°C Histoplasma capsulatum

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
25 37 activity at 37°C 2-fold higher than at 25°C Histoplasma capsulatum

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
-
 Histoplasma capsulatum

Cofactor

Cofactor Comment Organism Structure
FAD 1 mol of enzyme contains about 0.1 mol of flavin Histoplasma capsulatum