Activating Compound | Comment | Organism | Structure |
---|---|---|---|
cysteamine | at a reaction time of 45 min, CDO activity increases almost 20times in the presence of 5.8 mM cysteamine | Rattus norvegicus |
Cloned (Comment) | Organism |
---|---|
expressed in BL21(D3)pLysS cells containing the pET-14b/CDO-ORF plasmid | Rattus norvegicus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
alpha-ketoglutarate | alpha-ketoglutarate inhibits cysteine dioxygenase with 50% inhibition at 6.8 mM | Rattus norvegicus | |
aspartic acid | aspartic acid decreases enzyme activity to 50% at a concentration of 1.5 mM. Replacing the sulfydryl by the uncharged hydroxyl group of serine does not affect enzyme activity. | Rattus norvegicus | |
azide | inhibits with a 50% activity reduction at 1.4 mM | Rattus norvegicus | |
cyanide | inhibits with a 50% activity reduction at 2.7 mM | Rattus norvegicus | |
Mercaptopropionic acid | mercaptopropionic acid at a concentration of 1.2 mM inhibits cysteine dioxygenase activity by 50% | Rattus norvegicus | |
additional information | iodoacetamide has no effect on the enzyme activity; S-methylcysteine does not inhibit cysteine dioxygenase | Rattus norvegicus | |
S-carboxymethylcysteine | S-carboxymethylcysteine exhibits 50% inhibition at a concentration of 2.3 mM | Rattus norvegicus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rattus norvegicus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
immobilized nickel affinity chromatography | Rattus norvegicus |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
L-cysteine + O2 = 3-sulfinoalanine | Upon binding substrate, the structure of the iron site is pertubed but is still consistent with six O/N donor, indicating that cysteine may bind to the iron center, but is not bound via the sulfur atom. | Rattus norvegicus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
liver | - |
Rattus norvegicus | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-cysteine + O2 | - |
Rattus norvegicus | 3-sulfino-L-alanine | - |
? | |
additional information | cysteamine does not serve as substrate, D-cysteine does not serve as substrate | Rattus norvegicus | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
CDO | - |
Rattus norvegicus |