Activating Compound | Comment | Organism | Structure |
---|---|---|---|
additional information | with pure cysteine dioxygenase no effect of addition of NAD+ to the cysteine diosygenase is found. | Rattus norvegicus |
Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli strain BL21(DE3) | Rattus norvegicus |
Protein Variants | Comment | Organism |
---|---|---|
additional information | recombinant cysteine dioxygenase protein with a thioredoxin sequence and 6 x His tagged, after purification the 6x His tag and the thioredoxin sequence are removed using factor Xa. | Rattus norvegicus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
L-cysteine | concentrations of cysteine of 2 mM and above are inhibitory in assays of purified cysteine dioxygenase | Rattus norvegicus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.45 | - |
L-cysteine | for purified protein | Rattus norvegicus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Fe2+ | additon of 0.2 or 0.3 mM Fe2+ yields near-optimal activity. Activity of fully purified enzyme in the absence of added Fe2+ is below 1% of that measured in the presence of 0.3 mM ferrous sulfate. | Rattus norvegicus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
23030 | - |
MALDI-MS | Rattus norvegicus |
23030 | - |
theoretical value, calculated from the primary sequence | Rattus norvegicus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rattus norvegicus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
1 ml HisTrap HP column, metal ion (nickel) affinity chromatography, gel filtration, MonoQ 4.6/100 PE ion exchange column. The thioredoxin/6x His cysteine dioxygenase fusion protein separates into two apparent isoforms that elute as two distinct peaks, one that elutes at 50 mM imidazole and one that elutes at 100 mM imidazole during metal ion affinity chromatograohy. The protein in the second peak has a specific activity, that is 50-60% less than that of the protein in the first peak. In contrast to peak 1 peak 2 elutes as two pronounced peaks (A and B) from the MonoQ column. The cysteine dioxygenase in peak A has no detectabel activity. In the the standard cysteine dioxygenase purification procedure, only the form from peak 1 is retained and further purified. | Rattus norvegicus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
liver | cDNA | Rattus norvegicus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
With Lineweaver-Burk plot Vmax is estimated to be 1.870 micromol cysteinesulfinate/min/mg | Rattus norvegicus |
1.357 | - |
- |
Rattus norvegicus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-cysteine + O2 | - |
Rattus norvegicus | 3-sulfino-L-alanine | - |
? |
Synonyms | Comment | Organism |
---|---|---|
CDO | - |
Rattus norvegicus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.012 | - |
L-cysteine | assay standart conditions are: the enzyme is incubated at 37°C in the presence of 62.5 mM Mes buffer (pH 6.1), 0.3 mM ferrous sulfate, 0.0125 mM bathocuproine disulfonate and 1.2 mM cysteine | Rattus norvegicus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5.8 | 6.2 | highest activity | Rattus norvegicus |