Cloned (Comment) | Organism |
---|---|
gene cdo, expression as N-terminal maltose-binding protein fusion protein in Escherichia coli strain BL21(DE3)RIPL | Mus musculus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Fe2+ | a a mononuclear nonheme iron(II)-dependent enzyme. It possesses both an unusual 3-His facial ligation sphere to the iron center and a rare Cys-Tyr crosslink near the active site | Mus musculus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-cysteine + O2 | Mus musculus | - |
3-sulfinoalanine | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Mus musculus | - |
gene cdo | - |
Purification (Comment) | Organism |
---|---|
recombinant N-terminal maltose-binding protein fusion enzyme from Escherichia coli strain BL21(DE3)RIPL by anion exchange and amylose affinity chromatography | Mus musculus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-cysteine + O2 | - |
Mus musculus | 3-sulfinoalanine | - |
? | |
additional information | no activity with selenocysteine | Mus musculus | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
CDO | - |
Mus musculus |
Fe(II) cysteine dioxygenase | - |
Mus musculus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
22 | - |
assay at room temperature | Mus musculus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
assay at | Mus musculus |
General Information | Comment | Organism |
---|---|---|
additional information | NO as a substrate analogue for O2 is used to prepare a species that mimics the geometric and electronic structures of an early reaction intermediate, analysis by magnetic circular dichroism, electron paramagnetic resonance, and electronic absorption spectroscopies as well as computational methods including density functional theory and semiempirical calculations, quantum mechanics/molecular mechanics calculations, overview. The NO adducts of Cys- and selenocysteine (Sec)-bound Fe(II)CDO exhibit virtually identical electronic properties | Mus musculus |
physiological function | cysteine dioxygenase is a mononuclear nonheme iron(II)-dependent enzyme critical for maintaining appropriate cysteine and taurine levels in eukaryotic systems | Mus musculus |