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Literature summary for 1.13.11.20 extracted from

  • Wenning, L.; Stoeveken, N.; Wuebbeler, J.H.; Steinbuechel, A.
    Substrate and cofactor range differences of two cysteine dioxygenases from Ralstonia eutropha H16 (2015), Appl. Environ. Microbiol., 82, 910-921 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.13.11.20

Activating Compound

Activating Compound Comment Organism Structure
ethylxanthate 0.002 mM, 110% of initial activity, 0.004 mM, 89% of initial activity Cupriavidus necator

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Cupriavidus necator
gene H16_A1614, located on chromosome 1, DNA and amino acid sequence determination and analysis, phylogenetic analysis, recombinant expression of His-tagged enzyme CdoB in Escherichia coli Cupriavidus necator
gene H16_B1863, located on chromosome 2, DNA and amino acid sequence determination and analysis, phylogenetic analysis, recombinant expression of His-tagged enzyme CdoA in Escherichia coli. Escherichia coli cells expressing CdoA can convert 3-mercaptopropionate to 3-sulfinopropionate Cupriavidus necator

Inhibitors

Inhibitors Comment Organism Structure
2-mercaptoethanol
-
 Cupriavidus necator
3-Mercaptopropionate complete inhibition at 0.05 mM Cupriavidus necator
cysteamine
-
 Cupriavidus necator
EDTA 0.05 mM, 80% of initial activity, 0.1 mM, 61% of initial activity; 40% inhibition at 0.1 mM, 65% inhibition at 0.2 mM; complete inhibition at 0.05 mM Cupriavidus necator
ethyl xanthate 44% inhibition at 0.004 mM Cupriavidus necator
ethylxanthate 0.002 mM, 110% of initial activity, 0.004 mM, 89% of initial activity Cupriavidus necator
additional information metal ions are inhibitory above 0.2 mM Cupriavidus necator
sodium ethylxanthate 11% inhibition at 0.004 mM Cupriavidus necator

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.85
-
 L-cysteine pH 6.5, 30°C, recombinant His-tagged enzyme Cupriavidus necator
5.7
-
 3-Mercaptopropionate pH 6.5, 30°C, recombinant His-tagged enzyme Cupriavidus necator
6.7
-
 3-Mercaptopropanoate pH 6.5, 30°C Cupriavidus necator
6.7
-
 L-cysteine pH 6.5, 30°C, recombinant His-tagged enzyme Cupriavidus necator

Metals/Ions

Metals/Ions Comment Organism Structure
Cu2+ activates Cupriavidus necator
Fe2+ 0.38 mol Fe(II) per mol CdoB, activates Cupriavidus necator
Fe2+ 0.42 mol Fe(II) per mol CdoB, activates Cupriavidus necator
Fe3+ 0.048 mol Fe(III) per mol enzyme Cupriavidus necator
Iron iron content of 0.38 mol Fe(II) per mol of enzyme Cupriavidus necator
additional information CdoA contains no Fe3+ Cupriavidus necator
additional information metal ions are inhibitory above 0.2 mM Cupriavidus necator
Ni2+ activates Cupriavidus necator
Zn2+ activates Cupriavidus necator

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
46800
-
 gel filtration Cupriavidus necator
63900
-
 recombinant His-tagged enzyme CdoB, gel filtration Cupriavidus necator

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
L-cysteine + O2 Cupriavidus necator
-
 3-sulfinoalanine
-
 ?
L-cysteine + O2 Cupriavidus necator DSM 428
-
 3-sulfinoalanine
-
 ?
L-cysteine + O2 Cupriavidus necator H16 / ATCC 23440 / NCIB 10442 / S-10-1
-
 3-sulfinoalanine
-
 ?

Organism

Organism UniProt Comment Textmining
Cupriavidus necator Q0K029
-
-
Cupriavidus necator Q0K029 cf. EC 1.13.11.91
-
Cupriavidus necator Q0KB75
-
-
Cupriavidus necator DSM 428 Q0K029 cf. EC 1.13.11.91
-
Cupriavidus necator H16 / ATCC 23440 / NCIB 10442 / S-10-1 Q0K029
-
-
Cupriavidus necator H16 / ATCC 23440 / NCIB 10442 / S-10-1 Q0KB75
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant His-tagged enzyme CdoA from Escherichia coli by nickel affinity chromatography to homogeneity Cupriavidus necator
recombinant His-tagged enzyme CdoB from Escherichia coli by nickel affinity chromatography to homogeneity Cupriavidus necator

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.05
-
 pH 6.5, 30°C Cupriavidus necator

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3-mercaptopropanoate + O2 reaction of EC 1.13.11.91 Cupriavidus necator 3-sulfinopropanoate + H+
-
 ?
3-mercaptopropanoate + O2 reaction of EC 1.13.11.91 Cupriavidus necator DSM 428 3-sulfinopropanoate + H+
-
 ?
3-mercaptopropanoate + O2 reaction of EC 1.13.11.91 Cupriavidus necator H16 / ATCC 23440 / NCIB 10442 / S-10-1 3-sulfinopropanoate + H+
-
 ?
3-mercaptopropionate + O2 3fold higher activity compared to L-cysteine. 3-Mercaptopropionate does not occur naturally in cells of strain H16 Cupriavidus necator 3-sulfinopropionate
-
 ?
3-mercaptopropionate + O2 3fold higher activity compared to L-cysteine. 3-Mercaptopropionate does not occur naturally in cells of strain H16 Cupriavidus necator DSM 428 3-sulfinopropionate
-
 ?
3-mercaptopropionate + O2 3fold higher activity compared to L-cysteine. 3-Mercaptopropionate does not occur naturally in cells of strain H16 Cupriavidus necator H16 / ATCC 23440 / NCIB 10442 / S-10-1 3-sulfinopropionate
-
 ?
L-cysteine + O2
-
 Cupriavidus necator 3-sulfinoalanine
-
 ?
L-cysteine + O2
-
 Cupriavidus necator DSM 428 3-sulfinoalanine
-
 ?
L-cysteine + O2
-
 Cupriavidus necator H16 / ATCC 23440 / NCIB 10442 / S-10-1 3-sulfinoalanine
-
 ?
additional information no activity with 3-mercaptopropionate by CdoB Cupriavidus necator ?
-
 ?
additional information 3-mercaptopropanoate is preferred over cysteine. No substrate: cysteamine Cupriavidus necator ?
-
-
additional information 3-mercaptopropanoate is preferred over cysteine. No substrate: cysteamine Cupriavidus necator DSM 428 ?
-
-
additional information 3-mercaptopropanoate is preferred over cysteine. No substrate: cysteamine Cupriavidus necator H16 / ATCC 23440 / NCIB 10442 / S-10-1 ?
-
-
additional information no activity with 3-mercaptopropionate by CdoB Cupriavidus necator H16 / ATCC 23440 / NCIB 10442 / S-10-1 ?
-
 ?

Subunits

Subunits Comment Organism
? x * 22580, sequence calculation Cupriavidus necator
dimer 2 * 22580, calculated from sequence Cupriavidus necator
trimer 3 * 22390, sequence calculation Cupriavidus necator

Synonyms

Synonyms Comment Organism
CdoA
-
 Cupriavidus necator
CdoB
-
 Cupriavidus necator
H16_A1614
-
 Cupriavidus necator
H16_B1863
-
 Cupriavidus necator

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
 assay at Cupriavidus necator

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.01
-
 3-Mercaptopropanoate pH 6.5, 30°C Cupriavidus necator
0.01
-
 L-cysteine pH 6.5, 30°C, recombinant His-tagged enzyme Cupriavidus necator
0.25
-
 L-cysteine pH 6.5, 30°C, recombinant His-tagged enzyme Cupriavidus necator
0.82
-
 3-Mercaptopropionate pH 6.5, 30°C, recombinant His-tagged enzyme Cupriavidus necator

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.5
-
-
 Cupriavidus necator
6.8
-
-
 Cupriavidus necator

pI Value

Organism Comment pI Value Maximum pI Value
Cupriavidus necator sequence calculation
-
 5.8
Cupriavidus necator calculated from sequence
-
 5.8
Cupriavidus necator sequence calculation
-
 7

General Information

General Information Comment Organism
malfunction deletion of cdoA might enable increased synthesis of polythioesters Cupriavidus necator
physiological function importance of CdoA for the metabolism of the sulfur compounds 3,3'-thiodipropionic acid and 3,3'-dithiodipropionic acid by further converting their degradation product, 3-mercaptopropionate Cupriavidus necator

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.002
-
 3-Mercaptopropanoate pH 6.5, 30°C Cupriavidus necator
0.002
-
 L-cysteine pH 6.5, 30°C, recombinant His-tagged enzyme Cupriavidus necator
0.087
-
 L-cysteine pH 6.5, 30°C, recombinant His-tagged enzyme Cupriavidus necator
0.144
-
 3-Mercaptopropionate pH 6.5, 30°C, recombinant His-tagged enzyme Cupriavidus necator