KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
11.4 | - |
L-cysteine | pH 8.0, 25°C | Azotobacter vinelandii | |
26 | - |
cysteamine | pH 8.0, 25°C | Azotobacter vinelandii |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Iron | iron content is 0.8 mol of iron per mol of MDO. Addition of NO to 3-mercaptopropanoate-bound enzyme quantitatively yields an iron-nitrosyl species with EPR features consistent with a mononuclear (FeNO)7 site | Azotobacter vinelandii |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Azotobacter vinelandii | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Azotobacter vinelandii |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
3-mercaptopropanoate + O2 | reaction of EC 1.13.11.91 | Azotobacter vinelandii | 3-sulfinopropanoate + H+ | - |
? | |
cysteamine + O2 | - |
Azotobacter vinelandii | ? | - |
? | |
L-cysteine + O2 | - |
Azotobacter vinelandii | 3-sulfinoalanine | - |
? | |
additional information | enzyme exhibits a specificity for 3-mercaptopropanoate nearly 2 orders of magnitude greater than those for cysteine | Azotobacter vinelandii | ? | - |
- |
Subunits | Comment | Organism |
---|---|---|
? | x * 22696, calculated from sequence, x * 23000, SDS-PAGE | Azotobacter vinelandii |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.29 | - |
cysteamine | pH 8.0, 25°C | Azotobacter vinelandii | |
1.2 | - |
L-cysteine | pH 8.0, 25°C | Azotobacter vinelandii |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
11 | - |
cysteamine | pH 8.0, 25°C | Azotobacter vinelandii | |
110 | - |
L-cysteine | pH 8.0, 25°C | Azotobacter vinelandii |