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Literature summary for 1.13.11.20 extracted from

  • Fellner, M.; Aloi, S.; Tchesnokov, E.P.; Wilbanks, S.M.; Jameson, G.N.
    Substrate and pH-dependent kinetic profile of 3-mercaptopropionate dioxygenase from Pseudomonas aeruginosa (2016), Biochemistry, 55, 1362-1371 .
    View publication on PubMed
Search for more literature for 1.13.11.20

Crystallization (Commentary)

Crystallization (Comment) Organism
structure of variant G95C Pseudomonas aeruginosa

Protein Variants

Protein Variants Comment Organism
G95C variant is able to form a cysteine-tyrosine crosslink homologous to that found in mammalian cysteine dioxygenases. Activity of this variant is severely impaired Pseudomonas aeruginosa

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
12
-
 3-Mercaptopropanoate wild-type, phosphate buffer pH 8.0, 37°C Pseudomonas aeruginosa
13
-
 3-Mercaptopropanoate wild-type, phosphate buffer pH 7.5, 37°C Pseudomonas aeruginosa
13
-
 3-Mercaptopropanoate wild-type, PIPES buffer pH 7.5, 37°C Pseudomonas aeruginosa
15
-
 3-Mercaptopropanoate wild-type, PIPES buffer pH 7.0, 37°C Pseudomonas aeruginosa
16
-
 3-Mercaptopropanoate wild-type, PIPES buffer pH 6.1, 37°C Pseudomonas aeruginosa
16
-
 3-Mercaptopropanoate wild-type, PIPES buffer pH 6.5, 37°C Pseudomonas aeruginosa
22
-
 3-Mercaptopropanoate mutant G95C, PIPES buffer pH 6.5, 37°C Pseudomonas aeruginosa

Organism

Organism UniProt Comment Textmining
Pseudomonas aeruginosa Q9I0N5 cf. EC 1.13.11.91
-
Pseudomonas aeruginosa DSM 22644 Q9I0N5 cf. EC 1.13.11.91
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3-mercaptopropanoate + O2 reaction of EC 1.13.11.91 Pseudomonas aeruginosa 3-sulfinopropanoate + H+
-
 ?
3-mercaptopropanoate + O2 reaction of EC 1.13.11.91 Pseudomonas aeruginosa DSM 22644 3-sulfinopropanoate + H+
-
 ?
L-cysteine + O2
-
 Pseudomonas aeruginosa 3-sulfinoalanine
-
 ?
L-cysteine + O2
-
 Pseudomonas aeruginosa DSM 22644 3-sulfinoalanine
-
 ?

Synonyms

Synonyms Comment Organism
NP_251292
-
 Pseudomonas aeruginosa

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.003
-
 3-Mercaptopropanoate mutant G95C, PIPES buffer pH 6.5, 37°C Pseudomonas aeruginosa
0.011
-
 3-Mercaptopropanoate wild-type, PIPES buffer pH 6.1, 37°C Pseudomonas aeruginosa
0.013
-
 3-Mercaptopropanoate wild-type, phosphate buffer pH 8.0, 37°C Pseudomonas aeruginosa
0.019
-
 3-Mercaptopropanoate wild-type, PIPES buffer pH 7.5, 37°C Pseudomonas aeruginosa
0.021
-
 3-Mercaptopropanoate wild-type, phosphate buffer pH 7.5, 37°C Pseudomonas aeruginosa
0.022
-
 3-Mercaptopropanoate wild-type, PIPES buffer pH 6.5, 37°C Pseudomonas aeruginosa
0.033
-
 3-Mercaptopropanoate wild-type, PIPES buffer pH 7.0, 37°C Pseudomonas aeruginosa

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
additional information
-
 the monoprotonated ES complexes with 3-mercaptopropionic acid and cysteine have different pKs. At higher pH, kcat decreases sigmoidally with a similar pK regardless of substrate. Loss of reactivity at high pH is attributed to deprotonation of tyrosine 159 and its influence on dioxygen binding Pseudomonas aeruginosa

General Information

General Information Comment Organism
physiological function the monoprotonated ES complexes with 3-mercaptopropionic acid and cysteine have different pKs. At higher pH, kcat decreases sigmoidally with a similar pK regardless of substrate. Loss of reactivity at high pH is attributed to deprotonation of tyrosine 159 and its influence on dioxygen binding. A mechanism model shows deprotonation of tyrosine 159 both blocks oxygen binding and concomitantly promotes cystine formation Pseudomonas aeruginosa

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.7
-
 3-Mercaptopropanoate wild-type, PIPES buffer pH 6.1, 37°C Pseudomonas aeruginosa
1.1
-
 3-Mercaptopropanoate wild-type, phosphate buffer pH 8.0, 37°C Pseudomonas aeruginosa
1.4
-
 3-Mercaptopropanoate wild-type, PIPES buffer pH 6.5, 37°C Pseudomonas aeruginosa
1.5
-
 3-Mercaptopropanoate wild-type, PIPES buffer pH 7.5, 37°C Pseudomonas aeruginosa
1.6
-
 3-Mercaptopropanoate wild-type, phosphate buffer pH 7.5, 37°C Pseudomonas aeruginosa
2.2
-
 3-Mercaptopropanoate wild-type, PIPES buffer pH 7.0, 37°C Pseudomonas aeruginosa