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Literature summary for 1.13.11.25 extracted from

  • Tai, H.H.; Sih, C.J.
    3,4-Dihydroxy-9,10-secoandrosta-1,3,5(10)-triene-9,17-dione 4,5-dioxygenase from Nocardia restrictus. I. Isolation of the enzyme and study of its physical and chemical properties (1970), J. Biol. Chem., 245, 5062-5071.
    View publication on PubMed

Inhibitors

Inhibitors Comment Organism Structure
2,2'-dipyridyl
-
Rhodococcus equi
8-hydroxyquinoline
-
Rhodococcus equi
o-phenanthroline
-
Rhodococcus equi

Metals/Ions

Metals/Ions Comment Organism Structure
Fe2+ 1.13 gatom per mol of enzyme, stimulation of reaction Rhodococcus equi

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
274000
-
sedimentation equilibrium Rhodococcus equi
286000
-
sedimentation velocity Rhodococcus equi

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3,4-dihydroxy-9,10-secoandrosta-1,3,5(10)-triene-9,17-dione + O2 Rhodococcus equi
-
3-hydroxy-5,9,17-trioxo-4,5:9,10-disecoandrosta-1(10),2-dien-4-oate
-
?

Organism

Organism UniProt Comment Textmining
Rhodococcus equi
-
-
-

Purification (Commentary)

Purification (Comment) Organism
acetone, freezing and thawing, DEAE-cellulose, calcium phosphate cellulose Rhodococcus equi

Specific Activity [micromol/min/mg]

Specific Activity Minimum [Āµmol/min/mg] Specific Activity Maximum [Āµmol/min/mg] Comment Organism
22.17
-
-
Rhodococcus equi

Storage Stability

Storage Stability Organism
-20Ā°C, 10% acetone, relatively long perids, no loss of activity Rhodococcus equi

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3,4-dihydroxy-9,10-secoandrosta-1,3,5(10)-triene-9,17-dione + O2
-
Rhodococcus equi 3-hydroxy-5,9,17-trioxo-4,5:9,10-disecoandrosta-1(10),2-dien-4-oate
-
?
3-methylcatechol 7.4% of activity with 3-isopropylcatechol Rhodococcus equi ?
-
?
4-methylcatechol 4.3% of activity with 3-isopropylcatechol Rhodococcus equi ?
-
?

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5.8 7.5
-
Rhodococcus equi

pH Range

pH Minimum pH Maximum Comment Organism
4.5
-
inactive below Rhodococcus equi