BRENDA - Enzyme Database show
show all sequences of 1.13.11.26

Separation of tryptophan pyrrolooxygenase into three molecular forms. A study of their substrate specificities using tryptophyl-containing peptides and proteins

Camoretti-Mercado, B.; Frydman, R.B.; Eur. J. Biochem. 156, 317-325 (1986)

Data extracted from this reference:

Inhibitors
Inhibitors
Commentary
Organism
Structure
2-mercaptoethanol
2 mM, total activity loss
Triticum aestivum
alpha,alpha'-dipyridyl
-
Triticum aestivum
dithioerythritol
2 mM, total activity loss
Triticum aestivum
dithiothreitol
2 mM, total activity loss
Triticum aestivum
EDTA
-
Triticum aestivum
o-phenanthroline
-
Triticum aestivum
p-hydroxymercurisulfonate
10 mM, activity loss of 60%
Triticum aestivum
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
25000
-
molecular form A, SDS-PAGE
Triticum aestivum
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
L-tryptophan + O2
Triticum aestivum
-
formylkynurenine
-
-
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Triticum aestivum
-
-
-
Purification (Commentary)
Commentary
Organism
three molecular forms: A,B,C
Triticum aestivum
Source Tissue
Source Tissue
Commentary
Organism
Textmining
germ
-
Triticum aestivum
-
Storage Stability
Storage Stability
Organism
4C, forms A and C stable for at least two weeks, form B activity loss after three days
Triticum aestivum
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
D-tryptophan + O2
-
439517
Triticum aestivum
formylkynurenine
-
-
-
?
DL-5-fluorotryptophan + O2
-
439517
Triticum aestivum
5-fluoroformylkynurenine
-
-
-
?
DL-5-methyltryptophan + O2
-
439517
Triticum aestivum
5-methyl-N-formylkynurenine
-
-
-
?
DL-6-fluorotryptophan + O2
-
439517
Triticum aestivum
4-fluoro-N-formylkynurenine
-
-
-
?
DL-6-methyltryptophan + O2
-
439517
Triticum aestivum
4-methyl-N-formylkynurenine
-
-
-
?
DL-7-methyltryptophan + O2
-
439517
Triticum aestivum
3-methyl-N-formylkynurenine
-
-
-
?
DL-tryptophan + O2
-
439517
Triticum aestivum
formylkynurenine
-
-
-
?
DL-tryptophan methyl ester + O2
-
439517
Triticum aestivum
formylkynurenine methyl ester
-
-
-
?
L-5-hydroxytryptophan + O2
very poor substrate activity with molecular form A
439517
Triticum aestivum
5-hydroxy-N-formylkynurenine
-
-
-
?
L-tryptophan + O2
-
439517
Triticum aestivum
formylkynurenine
-
-
-
?
N-acetyl-5-methyltryptophan + O2
-
439517
Triticum aestivum
5-methyl-Nalpha-acetyl-Nomega-formylkynurenine
-
-
-
?
N-acetyl-DL-tryptophan + O2
-
439517
Triticum aestivum
N-acetylformylkynurenine
-
-
-
?
Temperature Optimum [C]
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
22
-
form C with chymotrypsin as substrate
Triticum aestivum
37
45
form A, tryptophan or gamma-chymotrypsin as substrates
Triticum aestivum
37
-
form B, form C with tryptophan as substrate
Triticum aestivum
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
8.6
-
in Tris-HCl buffer
Triticum aestivum
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
2-mercaptoethanol
2 mM, total activity loss
Triticum aestivum
alpha,alpha'-dipyridyl
-
Triticum aestivum
dithioerythritol
2 mM, total activity loss
Triticum aestivum
dithiothreitol
2 mM, total activity loss
Triticum aestivum
EDTA
-
Triticum aestivum
o-phenanthroline
-
Triticum aestivum
p-hydroxymercurisulfonate
10 mM, activity loss of 60%
Triticum aestivum
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
25000
-
molecular form A, SDS-PAGE
Triticum aestivum
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
L-tryptophan + O2
Triticum aestivum
-
formylkynurenine
-
-
?
Purification (Commentary) (protein specific)
Commentary
Organism
three molecular forms: A,B,C
Triticum aestivum
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
germ
-
Triticum aestivum
-
Storage Stability (protein specific)
Storage Stability
Organism
4C, forms A and C stable for at least two weeks, form B activity loss after three days
Triticum aestivum
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
D-tryptophan + O2
-
439517
Triticum aestivum
formylkynurenine
-
-
-
?
DL-5-fluorotryptophan + O2
-
439517
Triticum aestivum
5-fluoroformylkynurenine
-
-
-
?
DL-5-methyltryptophan + O2
-
439517
Triticum aestivum
5-methyl-N-formylkynurenine
-
-
-
?
DL-6-fluorotryptophan + O2
-
439517
Triticum aestivum
4-fluoro-N-formylkynurenine
-
-
-
?
DL-6-methyltryptophan + O2
-
439517
Triticum aestivum
4-methyl-N-formylkynurenine
-
-
-
?
DL-7-methyltryptophan + O2
-
439517
Triticum aestivum
3-methyl-N-formylkynurenine
-
-
-
?
DL-tryptophan + O2
-
439517
Triticum aestivum
formylkynurenine
-
-
-
?
DL-tryptophan methyl ester + O2
-
439517
Triticum aestivum
formylkynurenine methyl ester
-
-
-
?
L-5-hydroxytryptophan + O2
very poor substrate activity with molecular form A
439517
Triticum aestivum
5-hydroxy-N-formylkynurenine
-
-
-
?
L-tryptophan + O2
-
439517
Triticum aestivum
formylkynurenine
-
-
-
?
N-acetyl-5-methyltryptophan + O2
-
439517
Triticum aestivum
5-methyl-Nalpha-acetyl-Nomega-formylkynurenine
-
-
-
?
N-acetyl-DL-tryptophan + O2
-
439517
Triticum aestivum
N-acetylformylkynurenine
-
-
-
?
Temperature Optimum [C] (protein specific)
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
22
-
form C with chymotrypsin as substrate
Triticum aestivum
37
45
form A, tryptophan or gamma-chymotrypsin as substrates
Triticum aestivum
37
-
form B, form C with tryptophan as substrate
Triticum aestivum
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
8.6
-
in Tris-HCl buffer
Triticum aestivum
Other publictions for EC 1.13.11.26
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [C]
Temperature Range [C]
Temperature Stability [C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [C] (protein specific)
Temperature Range [C] (protein specific)
Temperature Stability [C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
439525
Walsh
Influence of the antidepressan ...
Rattus norvegicus
Life Sci.
62
2417-2423
1998
-
-
-
-
-
-
2
-
-
-
-
-
-
3
-
-
-
-
-
2
-
-
2
-
1
-
-
-
-
-
-
1
-
-
-
-
-
-
1
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
2
-
-
2
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
439517
Camoretti-Mercado
Separation of tryptophan pyrro ...
Triticum aestivum
Eur. J. Biochem.
156
317-325
1986
-
-
-
-
-
-
7
-
-
-
1
1
-
3
-
-
1
-
-
1
-
1
12
-
3
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
7
-
-
-
-
1
1
-
-
-
1
-
1
-
1
12
-
3
-
-
-
1
-
-
-
-
-
-
-
-
-
439518
Sburlati
Changes in the activities of p ...
Triticum aestivum
Plant Physiol.
71
822-827
1983
-
-
-
-
-
-
-
-
-
-
-
1
-
2
-
-
1
-
-
3
1
-
3
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
1
-
3
1
-
3
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
439519
Frydman
-
Pyrrolooxygenases from pepper ...
Capsicum annuum, Euphorbia pulcherrima
Phytochemistry
18
1119-1123
1979
-
-
-
-
-
-
-
-
-
-
-
1
-
2
-
-
-
-
-
2
-
-
1
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
2
-
-
1
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
439520
Mendy
-
Pyrrolooxygenases from argenti ...
Triticum aestivum
Phytochemistry
18
1427-1431
1979
-
-
-
-
-
-
-
-
-
-
-
1
-
1
-
-
-
-
-
2
1
-
1
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
2
1
-
1
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
439521
Frydman
Pyrrolooxygenases: a new type ...
Triticum aestivum
Mol. Cell. Biochem.
2
121-135
1973
-
-
-
-
-
-
-
2
-
-
-
1
-
1
-
-
-
-
-
1
1
-
2
-
-
-
1
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
1
-
-
-
-
-
1
1
-
2
-
-
-
1
-
1
-
-
-
-
-
-
-
-
-
439522
Frydman
Pyrrolooxygenases: isolation, ...
Triticum aestivum
Biochim. Biophys. Acta
284
63-79
1972
-
-
-
-
-
-
5
2
-
4
-
1
-
1
-
-
1
-
-
1
1
1
6
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
5
-
2
-
4
-
1
-
-
-
1
-
1
1
1
6
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
439523
Frydman
Pyrrolooxygenase: its action o ...
Triticum aestivum
Biochim. Biophys. Acta
284
80-89
1972
-
-
-
-
-
-
1
-
-
-
-
-
-
1
-
-
-
-
-
1
-
-
1
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
1
-
-
1
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
439524
Frydman
Pyrrolooxygenase: a new type o ...
Triticum aestivum
FEBS Lett.
15
305-308
1971
-
-
-
-
-
-
5
-
-
-
-
-
-
1
-
-
1
-
-
1
-
1
4
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
5
-
-
-
-
-
-
-
-
-
1
-
1
-
1
4
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-