Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 1.13.11.27 extracted from

  • Garcia, I.; Rodgers, M.; Pepin, R.; Hsieh, T.F.; Matringe, M.
    Characterization and subcellular compartmentation of recombinant 4-hydroxyphenylpyruvate dioxygenase from Arabidopsis in transgenic tobacco (1999), Plant Physiol., 119, 1507-1516.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
overproduction of the recombinant enzyme in Escherichia coli JM105, overexpression of the complete coding sequence in transgenic Nicotiana tabacum using Agrobacterium tumefaciens transformation Arabidopsis sp.

Localization

Localization Comment Organism GeneOntology No. Textmining
cytosol
-
Arabidopsis sp. 5829
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
100000
-
non-denaturing PAGE Arabidopsis sp.

Organism

Organism UniProt Comment Textmining
Arabidopsis sp.
-
-
-

Reaction

Reaction Comment Organism Reaction ID
4-hydroxyphenylpyruvate + O2 = homogentisate + CO2 in plants this enzyme activity is involved in two distinct metabolic processes, the biosynthesis of prenylquinones and the catabolism of tyrosine Arabidopsis sp.

Source Tissue

Source Tissue Comment Organism Textmining
leaf photosynthetically active tissue Arabidopsis sp.
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-hydroxyphenylpyruvate + O2
-
Arabidopsis sp. homogentisate + CO2
-
?

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.5
-
-
Arabidopsis sp.

pH Range

pH Minimum pH Maximum Comment Organism
5.5 7.7 pH 5.5: about 50% of maximal activity, pH 7.7: about 55% of maximal activity Arabidopsis sp.