Cloned (Comment) | Organism |
---|---|
overproduction of the recombinant enzyme in Escherichia coli JM105, overexpression of the complete coding sequence in transgenic Nicotiana tabacum using Agrobacterium tumefaciens transformation | Arabidopsis sp. |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytosol | - |
Arabidopsis sp. | 5829 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
100000 | - |
non-denaturing PAGE | Arabidopsis sp. |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Arabidopsis sp. | - |
- |
- |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
4-hydroxyphenylpyruvate + O2 = homogentisate + CO2 | in plants this enzyme activity is involved in two distinct metabolic processes, the biosynthesis of prenylquinones and the catabolism of tyrosine | Arabidopsis sp. |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
leaf | photosynthetically active tissue | Arabidopsis sp. | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-hydroxyphenylpyruvate + O2 | - |
Arabidopsis sp. | homogentisate + CO2 | - |
? |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.5 | - |
- |
Arabidopsis sp. |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
5.5 | 7.7 | pH 5.5: about 50% of maximal activity, pH 7.7: about 55% of maximal activity | Arabidopsis sp. |