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Literature summary for 1.13.11.27 extracted from
- Heterologous production of pyomelanin biopolymer using 4-hydroxyphenylpyruvate dioxygenase isolated from Ralstonia pickettii in Escherichia coli (2020), Biochem. Eng. J., 157, 107548 .
No PubMed abstract available
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression in Escherichia coli | Ralstonia pickettii |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| synthesis | recombinant Escherichia coli expression HppD can produce pyomelanin at levels up to 315.5 mg/l (13.1 mg/l/h) in 24 h. Highest pyomelanin production is achieved when L-tyrosine and 0.5mM Cu2+ are supplemented into LB medium | Ralstonia pickettii |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| soluble | - |
Ralstonia pickettii | - |
- |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Ralstonia pickettii | - |
- |
- |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 4-hydroxyphenylpyruvate + O2 | - |
Ralstonia pickettii | homogentisate + CO2 | - |
? |  |
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Release 2023.1 (January 2023)
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