Literature summary for 1.13.11.27 extracted from

Liu, B.; Peng, Q.; Sheng, M.; Ni, H.; Xiao, X.; Tao, Q.; He, Q.; He, J.
Isolation and characterization of a topramezone-resistant 4-hydroxyphenylpyruvate dioxygenase from Sphingobium sp. TPM-19 (2020), J. Agric. Food Chem., 68, 1022-1029 .

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Cloned(Commentary)

Cloned (Comment)	Organism
expression in Escherichia coli	Sphingobium sp.

Inhibitors

Inhibitors	Comment	Organism	Structure
DEPC	1 mM, no residual activity	Sphingobium sp.
EDTA	1 mM, no residual activity	Sphingobium sp.
isoxaflutole	-	Sphingobium sp.
mesotrione	-	Sphingobium sp.
additional information	not inhibitory at 1 mM: urea, PMSF	Sphingobium sp.
tembotrione	-	Sphingobium sp.
topramezone	-	Sphingobium sp.

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.083	-	4-hydroxyphenylpyruvate	pH 6, 35°C	Sphingobium sp.

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Ca2+	66.5% of the activity with Fe2+	Sphingobium sp.
Fe2+	required	Sphingobium sp.
Fe3+	95% of the reactivity with Fe2+	Sphingobium sp.
Mg2+	101% of the activity with Fe2+	Sphingobium sp.
Mn2+	22% of the activity with Fe2+	Sphingobium sp.

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
40000	-	gel filtration	Sphingobium sp.

Organism

Organism	UniProt	Comment	Textmining
Sphingobium sp.	A0A6B9L0W0	-	-

Purification (Commentary)

Purification (Comment)	Organism
recombinant enzyme, using Co2+-affinity chromatography	Sphingobium sp.

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
4-hydroxyphenylpyruvate + O2	-	Sphingobium sp.	homogentisate + CO2	-	?

Subunits

Subunits	Comment	Organism
monomer	1 * 41000, SDS-PAGE, 1 * 38800, calculated from sequence	Sphingobium sp.

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
15	-	4-hydroxyphenylpyruvate	pH 6, 35°C	Sphingobium sp.

Ki Value [mM]

Ki Value [mM]	Ki Value maximum [mM]	Inhibitor	Comment	Organism	Structure
0.001	-	tembotrione	pH 6, 35°C	Sphingobium sp.
0.0013	-	mesotrione	pH 6, 35°C	Sphingobium sp.
0.0025	-	topramezone	pH 6, 35°C	Sphingobium sp.
0.006	-	isoxaflutole	pH 6, 35°C	Sphingobium sp.

IC50 Value

IC50 Value	IC50 Value Maximum	Comment	Organism	Inhibitor	Structure
0.0025	-	pH 6, 35°C	Sphingobium sp.	tembotrione
0.0042	-	pH 6, 35°C	Sphingobium sp.	mesotrione
0.0052	-	pH 6, 35°C	Sphingobium sp.	topramezone
0.0087	-	pH 6, 35°C	Sphingobium sp.	isoxaflutole

kcat/KM [mM/s]

kcat/KM Value [1/mMs^-1]	kcat/KM Value Maximum [1/mMs^-1]	Substrate	Comment	Organism	Structure
181.2	-	4-hydroxyphenylpyruvate	pH 6, 35°C	Sphingobium sp.

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Release 2023.1 (January 2023)