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Literature summary for 1.13.11.27 extracted from

  • Feng, A.N.; Huang, C.W.; Lin, C.H.; Chang, Y.L.; Ni, M.Y.; Lee, H.J.
    Role of the N-terminus in human 4-hydroxyphenylpyruvate dioxygenase activity (2020), J. Biochem., 167, 315-322 .
    View publication on PubMed

Protein Variants

Protein Variants Comment Organism
E12A 2fold decrease in catalytic efficiency Homo sapiens
K10A 2fold decrease in catalytic efficiency Homo sapiens
additional information upon removal of the 12 N-terminal residues, the kcat/Km decreases 8fold compared to wild-type. The wild-type level of activity is retained in a mutant missing the 17 N-terminal residues, with a kcat/Km 11fold higher than that of the mutant lacking 12 N-terminal residues, however, the structural stability of this mutant is lower than that of wild type Homo sapiens
P11A 30% increase in catalytic efficiency Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.1
-
4-hydroxyphenylpyruvate mutant K10A, pH 6.5, 37°C Homo sapiens
0.1
-
4-hydroxyphenylpyruvate mutant lacking 17 N-terminal residues, pH 6.5, 37°C Homo sapiens
0.18
-
4-hydroxyphenylpyruvate mutant E12A, pH 6.5, 37°C Homo sapiens
0.18
-
4-hydroxyphenylpyruvate mutant P11A, pH 6.5, 37°C Homo sapiens
0.24
-
4-hydroxyphenylpyruvate wild-type, pH 6.5, 37°C Homo sapiens
0.37
-
4-hydroxyphenylpyruvate mutant lacking 12 N-terminal residues, pH 6.5, 37°C Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens P32754
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-hydroxyphenylpyruvate + O2
-
Homo sapiens homogentisate + CO2
-
?

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
54.4
-
melting temperature, mutant lacking 17 N-terminal residues Homo sapiens
56.5
-
melting temperature, mutant lacking 12 N-terminal residues Homo sapiens
56.7
-
melting temperature, mutant E12A Homo sapiens
57.1
-
melting temperature, wild-type Homo sapiens
58
-
melting temperature, mutant P11A Homo sapiens
59.8
-
melting temperature, mutant K10A Homo sapiens

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.7
-
4-hydroxyphenylpyruvate mutant K10A, pH 6.5, 37°C Homo sapiens
0.7
-
4-hydroxyphenylpyruvate mutant lacking 12 N-terminal residues, pH 6.5, 37°C Homo sapiens
1.4
-
4-hydroxyphenylpyruvate mutant E12A, pH 6.5, 37°C Homo sapiens
2.2
-
4-hydroxyphenylpyruvate mutant lacking 17 N-terminal residues, pH 6.5, 37°C Homo sapiens
3.5
-
4-hydroxyphenylpyruvate mutant P11A, pH 6.5, 37°C Homo sapiens
3.7
-
4-hydroxyphenylpyruvate wild-type, pH 6.5, 37°C Homo sapiens

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
2
-
4-hydroxyphenylpyruvate mutant lacking 12 N-terminal residues, pH 6.5, 37°C Homo sapiens
8
-
4-hydroxyphenylpyruvate mutant E12A, pH 6.5, 37°C Homo sapiens
8
-
4-hydroxyphenylpyruvate mutant K10A, pH 6.5, 37°C Homo sapiens
15
-
4-hydroxyphenylpyruvate wild-type, pH 6.5, 37°C Homo sapiens
20
-
4-hydroxyphenylpyruvate mutant P11A, pH 6.5, 37°C Homo sapiens
22
-
4-hydroxyphenylpyruvate mutant lacking 17 N-terminal residues, pH 6.5, 37°C Homo sapiens