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Literature summary for 1.13.11.27 extracted from

  • Hawkes, T.R.; Langford, M.P.; Viner, R.; Blain, R.E.; Callaghan, F.M.; Mackay, E.A.; Hogg, B.V.; Singh, S.; Dale, R.P.
    Characterization of 4-hydroxyphenylpyruvate dioxygenases, inhibition by herbicides and engineering for herbicide tolerance in crops (2019), Pestic. Biochem. Physiol., 156, 9-28 .
    View publication on PubMed
Search for more literature for 1.13.11.27

Protein Variants

Protein Variants Comment Organism
A327R/L359M about 90% of wild-type activity Avena sativa
G411A about 15% of wild-type activity Avena sativa
G413W about 20% of wild-type activity Avena sativa
L359M about 80% of wild-type activity Avena sativa
P315R/R328A/L347M mutations induce an 8fold increase in the value of k4 in respect of mesotrione Arabidopsis thaliana
V218I/A327R/I340E/L359M about 165% of wild-type activity Avena sativa
V218I/A327R/I340E/L359M/G411A about 6% of wild-type activity Avena sativa
V218I/A327R/L359M about 80% of wild-type activity Avena sativa
V218L/A327R/I340E/L359M about 170% of wild-type activity Avena sativa

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0017
-
 4-hydroxyphenylpyruvate mutant V218L/A327R/I340E/L359M , pH 7, 25°C Avena sativa
0.002
-
 4-hydroxyphenylpyruvate mutant V218I/A327R/I340E/L359M, pH 7, 25°C Avena sativa
0.0026
-
 4-hydroxyphenylpyruvate mutant A327R/L359M, pH 7, 25°C Avena sativa
0.00304
-
 4-hydroxyphenylpyruvate pH 7, 25°C Arabidopsis thaliana
0.0031
-
 4-hydroxyphenylpyruvate mutant V218I/A327R/L359M, pH 7, 25°C Avena sativa
0.0043
-
 4-hydroxyphenylpyruvate mutant L359M, pH 7, 25°C Avena sativa
0.0048
-
 4-hydroxyphenylpyruvate wild-type, pH 7, 25°C Avena sativa
0.0143
-
 4-hydroxyphenylpyruvate mutant V218I/A327R/I340E/L359M/G411A, pH 7, 25°C Avena sativa
0.0309
-
 4-hydroxyphenylpyruvate mutant G413W, pH 7, 25°C Avena sativa
0.0389
-
 4-hydroxyphenylpyruvate mutant G411A, pH 7, 25°C Avena sativa

Metals/Ions

Metals/Ions Comment Organism Structure
Iron 0.37 atoms per protomer Avena sativa
Iron 0.37 atoms per protomer Arabidopsis thaliana

Organism

Organism UniProt Comment Textmining
Arabidopsis thaliana P93836
-
-
Avena sativa
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-hydroxyphenylpyruvate + O2
-
 Avena sativa homogentisate + CO2
-
 ?
4-hydroxyphenylpyruvate + O2
-
 Arabidopsis thaliana homogentisate + CO2
-
 ?

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.25
-
 4-hydroxyphenylpyruvate mutant V218I/A327R/I340E/L359M/G411A, pH 7, 25°C Avena sativa
3.52
-
 4-hydroxyphenylpyruvate mutant A327R/L359M, pH 7, 25°C Avena sativa
4.25
-
 4-hydroxyphenylpyruvate wild-type, pH 7, 25°C Arabidopsis thaliana
4.54
-
 4-hydroxyphenylpyruvate mutant V218I/A327R/L359M, pH 7, 25°C Avena sativa
4.54
-
 4-hydroxyphenylpyruvate mutant V218L/A327R/I340E/L359M , pH 7, 25°C Avena sativa
4.96
-
 4-hydroxyphenylpyruvate mutant G411A, pH 7, 25°C Avena sativa
4.99
-
 4-hydroxyphenylpyruvate mutant V218I/A327R/I340E/L359M, pH 7, 25°C Avena sativa
5.04
-
 4-hydroxyphenylpyruvate mutant L359M, pH 7, 25°C Avena sativa
6.97
-
 4-hydroxyphenylpyruvate mutant G413W, pH 7, 25°C Avena sativa
7.27
-
 4-hydroxyphenylpyruvate wild-type, pH 7, 25°C Avena sativa
8.25
-
 4-hydroxyphenylpyruvate mutant P315R/R328A/L347M, pH 7, 25°C Arabidopsis thaliana

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
90
-
 4-hydroxyphenylpyruvate mutant V218I/A327R/I340E/L359M/G411A, pH 7, 25°C Avena sativa
140
-
 4-hydroxyphenylpyruvate mutant G411A, pH 7, 25°C Avena sativa
230
-
 4-hydroxyphenylpyruvate mutant G413W, pH 7, 25°C Avena sativa
1210
-
 4-hydroxyphenylpyruvate mutant L359M, pH 7, 25°C Avena sativa
1370
-
 4-hydroxyphenylpyruvate mutant A327R/L359M, pH 7, 25°C Avena sativa
1410
-
 4-hydroxyphenylpyruvate pH 7, 25°C Arabidopsis thaliana
1570
-
 4-hydroxyphenylpyruvate wild-type, pH 7, 25°C Avena sativa
1610
-
 4-hydroxyphenylpyruvate mutant V218I/A327R/L359M, pH 7, 25°C Avena sativa
2510
-
 4-hydroxyphenylpyruvate mutant V218I/A327R/I340E/L359M, pH 7, 25°C Avena sativa
2660
-
 4-hydroxyphenylpyruvate mutant V218L/A327R/I340E/L359M , pH 7, 25°C Avena sativa