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Literature summary for 1.13.11.3 extracted from
- Protocatechuate 3,4-dioxygenase. I. Crystallization and characterization (1968), J. Biol. Chem., 243, 2673-2681.
Crystallization (Commentary)
| Crystallization (Comment) | Organism |
|---|---|
| addition of 0.1 ml 1 M 2-mercaptoethanol to 10 ml enzyme solution in 50 mM Tris buffer pH 8.5, rhombic crystals appear after 1 week in a refrigerator, crystallization is also achieved by adding 1 mM iodoacetamide instead of mercaptoethanol or by dialyzing the enzyme against cold, destilled water for 2 days | Pseudomonas aeruginosa |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 2,3-Dihydroxybenzoate | - |
Pseudomonas aeruginosa |  |
| 3,4-Dihydroxyacetophenone | - |
Pseudomonas aeruginosa | |
| 3,4-dihydroxyphenylacetic acid | - |
Pseudomonas aeruginosa | |
| Protocatechualdehyde | competitive inhibitor | Pseudomonas aeruginosa | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.03 | - |
protocatechuate | - |
Pseudomonas aeruginosa | |
| 0.043 | - |
O2 | - |
Pseudomonas aeruginosa | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Fe | enzyme contains 7.3 g-atom of nonheme iron per mol of enzyme | Pseudomonas aeruginosa | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 700000 | - |
diffusion data | Pseudomonas aeruginosa |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Pseudomonas aeruginosa | - |
nduced in cells grown on 4-hydroxybenzoate | - |
| Pseudomonas aeruginosa B-10 | - |
nduced in cells grown on 4-hydroxybenzoate | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Pseudomonas aeruginosa |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 61.6 | - |
- |
Pseudomonas aeruginosa |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 3,4-dihydroxybenzoate + O2 | very low activity with: 3,4-dihydroxyphenylacetic acid | Pseudomonas aeruginosa | 3-carboxy-cis,cis-muconate | - |
? | |
| 3,4-dihydroxybenzoate + O2 | very low activity with 3,4-dihydroxymandelic acid | Pseudomonas aeruginosa | 3-carboxy-cis,cis-muconate | - |
? | |
| 3,4-dihydroxybenzoate + O2 | very low activity with 4'-methylcatechol | Pseudomonas aeruginosa | 3-carboxy-cis,cis-muconate | - |
? | |
| 3,4-dihydroxybenzoate + O2 | very low activity with: 3,4-dihydroxyphenylacetic acid | Pseudomonas aeruginosa B-10 | 3-carboxy-cis,cis-muconate | - |
? | |
| 3,4-dihydroxybenzoate + O2 | very low activity with 3,4-dihydroxymandelic acid | Pseudomonas aeruginosa B-10 | 3-carboxy-cis,cis-muconate | - |
? | |
| 3,4-dihydroxybenzoate + O2 | very low activity with 4'-methylcatechol | Pseudomonas aeruginosa B-10 | 3-carboxy-cis,cis-muconate | - |
? | |
| 3-methylcatechol + O2 | at 0.4% the rate of protocatechuic acid oxidation | Pseudomonas aeruginosa | 2-methylmuconate | - |
? | |
| catechol + O2 | at 0.4% the rate of protocatechuic acid oxidation | Pseudomonas aeruginosa | muconate | - |
? | |
| catechol + O2 | at 0.4% the rate of protocatechuic acid oxidation | Pseudomonas aeruginosa B-10 | muconate | - |
? | |
| pyrogallol + O2 | at 0.4% the rate of protocatechuic acid oxidation | Pseudomonas aeruginosa | ? | - |
? | |
| pyrogallol + O2 | at 0.4% the rate of protocatechuic acid oxidation | Pseudomonas aeruginosa B-10 | ? | - |
? | |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 60 | - |
5 min, about 5% loss of activity | Pseudomonas aeruginosa |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 758 | - |
3,4-dihydroxybenzoate | - |
Pseudomonas aeruginosa | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8 | - |
- |
Pseudomonas aeruginosa |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 6 | 9.6 | 24°C, 1 week, less than 10% loss of activity | Pseudomonas aeruginosa |
Ki Value [mM]
| Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.014 | - |
Protocatechualdehyde | - |
Pseudomonas aeruginosa | |
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