Any feedback?
Literature summary for 1.13.11.3 extracted from
- Protocatechuate 3,4-dioxygenase from Brevibacterium fuscum (1990), Methods Enzymol., 188, 82-88.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| Na2SO4 | nonessential activator | Brevibacterium fuscum |  |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.125 | - |
protocatechuate | - |
Brevibacterium fuscum | |
| 0.8 | - |
O2 | - |
Brevibacterium fuscum | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Fe | 5 irons per enzyme molecule | Brevibacterium fuscum | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 22500 | - |
alpha,beta, 5 * 22500 + 5 * 40000, SDS-PAGE | Brevibacterium fuscum |
| 40000 | - |
alpha,beta, 5 * 22500 + 5 * 40000, SDS-PAGE | Brevibacterium fuscum |
| 315000 | - |
analytical ultracentrifugation | Brevibacterium fuscum |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 3,4-dihydroxybenzoate + O2 | Brevibacterium fuscum | - |
3-carboxy-cis,cis-muconate | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Brevibacterium fuscum | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| ammonium sulfate, DEAE-Sepharose, Phenyl-Sepharose | Brevibacterium fuscum |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 41 | - |
- |
Brevibacterium fuscum |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -20°C, stable for several years | Brevibacterium fuscum |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 3,4-dihydroxybenzoate + O2 | - |
Brevibacterium fuscum | 3-carboxy-cis,cis-muconate | - |
? | |
| 3,4-dihydroxybenzoate + O2 | absolute requirement for vicinal hydroxyl groups in the 3- and 4-position | Brevibacterium fuscum | 3-carboxy-cis,cis-muconate | - |
ir | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| decamer | alpha,beta, 5 * 22500 + 5 * 40000, SDS-PAGE | Brevibacterium fuscum |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 69.5 | - |
O2 | - |
Brevibacterium fuscum | |
| 417 | - |
3,4-dihydroxybenzoate | - |
Brevibacterium fuscum | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.8 | 8 | - |
Brevibacterium fuscum |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
