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Literature summary for 1.13.11.3 extracted from

  • Mohan, V.P.; Kishore, G.; Sugumaran, M.; Vaidyanathan, C.S.
    Purification and properties of protocatechuate-3,4-dioxygenase from Tecoma stans L. (1979), Plant Sci. Lett., 16, 267-272.
No PubMed abstract available

Inhibitors

Inhibitors Comment Organism Structure
4-hydroxymercuribenzoate 0.1 mM, complete inhibition Tecoma stans
iodoacetate 0.1 mM, complete inhibition Tecoma stans
N-ethylmaleimide 0.1 mM, complete inhibition Tecoma stans

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.04
-
FeSO4
-
Tecoma stans
0.33
-
protocatechuate
-
Tecoma stans

Localization

Localization Comment Organism GeneOntology No. Textmining
cytosol localized in 100000 g supernatant, completely absent in mitochondrial, microsomal and chloroplast fraction Tecoma stans 5829
-

Metals/Ions

Metals/Ions Comment Organism Structure
Fe external addition of FeSO4 is absolutely essential, other metal ions cannot replace Fe2+ Tecoma stans

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
150000
-
gel filtration Tecoma stans

Organism

Organism UniProt Comment Textmining
Tecoma stans
-
-
-

Purification (Commentary)

Purification (Comment) Organism
ammonium sulfate, DEAE-cellulose Tecoma stans

Source Tissue

Source Tissue Comment Organism Textmining
leaf
-
Tecoma stans
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.0081
-
-
Tecoma stans

Storage Stability

Storage Stability Organism
stable only 7 days Tecoma stans

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3,4-dihydroxybenzoate + O2 no other substrate found Tecoma stans 3-carboxy-cis,cis-muconate
-
?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
40
-
-
Tecoma stans

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
60
-
5 min, stable Tecoma stans

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5.2
-
completely inactive above pH 6.5 Tecoma stans