Inhibitors | Comment | Organism | Structure |
---|---|---|---|
4-hydroxymercuribenzoate | 0.1 mM, complete inhibition | Tecoma stans | |
iodoacetate | 0.1 mM, complete inhibition | Tecoma stans | |
N-ethylmaleimide | 0.1 mM, complete inhibition | Tecoma stans |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.04 | - |
FeSO4 | - |
Tecoma stans | |
0.33 | - |
protocatechuate | - |
Tecoma stans |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytosol | localized in 100000 g supernatant, completely absent in mitochondrial, microsomal and chloroplast fraction | Tecoma stans | 5829 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Fe | external addition of FeSO4 is absolutely essential, other metal ions cannot replace Fe2+ | Tecoma stans |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
150000 | - |
gel filtration | Tecoma stans |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Tecoma stans | - |
- |
- |
Purification (Comment) | Organism |
---|---|
ammonium sulfate, DEAE-cellulose | Tecoma stans |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
leaf | - |
Tecoma stans | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.0081 | - |
- |
Tecoma stans |
Storage Stability | Organism |
---|---|
stable only 7 days | Tecoma stans |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
3,4-dihydroxybenzoate + O2 | no other substrate found | Tecoma stans | 3-carboxy-cis,cis-muconate | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | - |
- |
Tecoma stans |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | - |
5 min, stable | Tecoma stans |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5.2 | - |
completely inactive above pH 6.5 | Tecoma stans |