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Literature summary for 1.13.11.3 extracted from

  • Durham, D.R.; Stirling, L.A.; Ornsten, L.N.; Perry, J.J.
    Intergeneric evolutionary homology revealed by the study of protocatechuate 3,4-dioxygenase from Azotobacter vinelandii (1980), Biochemistry, 19, 149-155.
    View publication on PubMed

Inhibitors

Inhibitors Comment Organism Structure
2,3-Dihydroxybenzoate
-
Azotobacter vinelandii
3,4-dihydroxyphenylacetic acid
-
Azotobacter vinelandii
4-(dimethylamino)benzaldehyde
-
Azotobacter vinelandii
4-hydroxybenzoate
-
Azotobacter vinelandii
4-methylcatechol
-
Azotobacter vinelandii
4-nitrocatechol
-
Azotobacter vinelandii
caffeic acid
-
Azotobacter vinelandii
catechol
-
Azotobacter vinelandii
Protocatechualdehyde
-
Azotobacter vinelandii

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.018
-
protocatechuate
-
Azotobacter vinelandii
0.0275
-
O2
-
Azotobacter vinelandii

Metals/Ions

Metals/Ions Comment Organism Structure
Fe 10 g-atoms per mol enzyme Azotobacter vinelandii

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
23300
-
alpha,beta, 5 * 23300 + 5 * 25500, SDS-PAGE Azotobacter vinelandii
25500
-
alpha,beta, 5 * 23300 + 5 * 25500, SDS-PAGE Azotobacter vinelandii
480000
-
ultracentrifugation Azotobacter vinelandii
510000
-
gel filtration Azotobacter vinelandii

Organism

Organism UniProt Comment Textmining
Azotobacter vinelandii
-
-
-

Purification (Commentary)

Purification (Comment) Organism
ammonium sulfate, DEAE-cellulose, Bio-gel agarose, DEAE-cellulose Azotobacter vinelandii

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
46.69
-
-
Azotobacter vinelandii

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3,4-dihydroxybenzoate + O2
-
Azotobacter vinelandii 3-carboxy-cis,cis-muconate
-
?
3-methylcatechol + O2 at 5% the rate of protocatechuic acid oxidation Azotobacter vinelandii 2-methylmuconate
-
?
catechol + O2 at 3% the rate of protocatechuic acid oxidation Azotobacter vinelandii muconate
-
?
pyrogallol + O2 at 10% the rate of protocatechuic acid oxidation Azotobacter vinelandii ?
-
?

Subunits

Subunits Comment Organism
decamer alpha,beta, 5 * 23300 + 5 * 25500, SDS-PAGE Azotobacter vinelandii

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
50
-
pH 8.5, 90 min, stable Azotobacter vinelandii
60
-
pH 8.5, 10 min, 90% loss of activity Azotobacter vinelandii

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.5
-
-
Azotobacter vinelandii

pH Stability

pH Stability pH Stability Maximum Comment Organism
8.5
-
50°C, 90 min stable Azotobacter vinelandii

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.0006
-
4-nitrocatechol
-
Azotobacter vinelandii
0.001
-
Protocatechualdehyde
-
Azotobacter vinelandii