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Literature summary for 1.13.11.3 extracted from

  • Zhang, L.S.; Fang, Y.; Zhou, Y.; Ye, B.C.
    Improvement of the Stabilization and activity of protocatechuate 3,4-dioxygenase isolated from Rhizobium sp. LMB-1 and immobilized on Fe3O4 nanoparticles (2017), Appl. Biochem. Biotechnol., 183, 1035-1048 .
    View publication on PubMed
Search for more literature for 1.13.11.3

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Rhizobium sp. LMB-1

General Stability

General Stability Organism
Fe3O4 nanoparticles-immobilized enzyme exhibits improved thermal stability compared to the free enzyme Rhizobium sp. LMB-1

Inhibitors

Inhibitors Comment Organism Structure
Al3+ 23.1% residual activity at 1 mM Rhizobium sp. LMB-1
butanol about 75% residual activity at 100 mM Rhizobium sp. LMB-1
ethanol about 50% residual activity at 100 mM Rhizobium sp. LMB-1
K+ 16.7% residual activity at 1 mM Rhizobium sp. LMB-1
methanol about 75% residual activity at 100 mM Rhizobium sp. LMB-1
Mg2+ 50% residual activity at 1 mM Rhizobium sp. LMB-1
Mn2+ 45.2% residual activity at 1 mM Rhizobium sp. LMB-1
Ni2+ 38.3% residual activity at 1 mM Rhizobium sp. LMB-1
Propanol about 40% residual activity at 100 mM Rhizobium sp. LMB-1

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.06534
-
 3,4-dihydroxybenzoate free enzyme, at pH 7.4 and 37°C Rhizobium sp. LMB-1
0.15929
-
 3,4-dihydroxybenzoate Fe3O4 nanoparticles-immobilized enzyme, at pH 7.4 and 37°C Rhizobium sp. LMB-1

Metals/Ions

Metals/Ions Comment Organism Structure
Fe3+ 115% activity at 1 mM Rhizobium sp. LMB-1

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
500000
-
 gel filtration Rhizobium sp. LMB-1

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3,4-dihydroxybenzoate + O2 Rhizobium sp. LMB-1
-
 3-carboxy-cis,cis-muconate
-
 ?

Organism

Organism UniProt Comment Textmining
Rhizobium sp. LMB-1 A0A193DXA9 and A0A193DXP2 subunit alpha and subunit beta
-

Purification (Commentary)

Purification (Comment) Organism
Ni-affinity column chromatography Rhizobium sp. LMB-1

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3,4-dihydroxybenzoate + O2
-
 Rhizobium sp. LMB-1 3-carboxy-cis,cis-muconate
-
 ?

Subunits

Subunits Comment Organism
heterodimer 1 * 23700 + 1 * 28600, SDS-PAGE Rhizobium sp. LMB-1

Synonyms

Synonyms Comment Organism
P34O
-
 Rhizobium sp. LMB-1

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
60
-
-
 Rhizobium sp. LMB-1

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
20 70 the enzyme shows more than 50% activity between 20 and 70°C Rhizobium sp. LMB-1

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
60 70 after incubating for 330 min at 60°C, the free enzyme maintains approximately 17% of its activity, and its half-life is 180 min. The enzyme retains 75% activity after 4 h at 70°C and shows no activity after 4 h at 90°C Rhizobium sp. LMB-1

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
10.25
-
 3,4-dihydroxybenzoate free enzyme, at pH 7.4 and 37°C Rhizobium sp. LMB-1
15.97
-
 3,4-dihydroxybenzoate Fe3O4 nanoparticles-immobilized enzyme, at pH 7.4 and 37°C Rhizobium sp. LMB-1

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
-
 Rhizobium sp. LMB-1

pH Range

pH Minimum pH Maximum Comment Organism
5.5 8.5 the enzyme shows more than 50% activity between pH 5.5 and 8.5 Rhizobium sp. LMB-1