Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 1.13.11.31 extracted from

  • Wecksler, A.T.; Jacquot, C.; van der Donk, W.A.; Holman, T.R.
    Mechanistic investigations of human reticulocyte 15- and platelet 12-lipoxygenases with arachidonic acid (2009), Biochemistry, 48, 6259-6267.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.13.11.31

Cloned(Commentary)

Cloned (Comment) Organism
overexpressed as N-terminally, His6-tagged protein Homo sapiens

Metals/Ions

Metals/Ions Comment Organism Structure
Fe2+
-
 Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Purification (Commentary)

Purification (Comment) Organism
to greater than 90% purity Homo sapiens

Source Tissue

Source Tissue Comment Organism Textmining
blood platelet
-
 Homo sapiens
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
arachidonate + O2
-
 Homo sapiens (5Z,8Z,10E,14Z)-(12S)-12-hydroperoxyicosa-5,8,10,14-tetraenoate
-
 ?

Synonyms

Synonyms Comment Organism
12-lipoxygenase
-
 Homo sapiens
12-LO
-
 Homo sapiens

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.4
-
 arachidonic acid at 15°C, pH 7.5 Homo sapiens
20
-
 arachidonic acid at 40°C, pH 7.5 Homo sapiens

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
1.4
-
 arachidonic acid at 15°C, pH 7.5 Homo sapiens
50
-
 arachidonic acid at 40°C, pH 7.5 Homo sapiens