Inhibitors | Comment | Organism | Structure |
---|---|---|---|
PD146176 | selective inhibitor of 12/15-LOX, macrophages treated with PD146176 elaborate reduced levels of interleukin-12 in response to Toxoplasma gondii antigen | Mus musculus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Mus musculus | - |
C57BL/6 mice | - |
Mus musculus C57BL/6 | - |
C57BL/6 mice | - |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
brain | 12/15-LOX is selectively enhanced in the periphery, 12/15-LOX-expressing myeloid cells are enriched in the brain during chronic toxoplasmosis | Mus musculus | - |
macrophage | 12/15-LOX is a macrophage-selective regulator of interleukin-12 production | Mus musculus | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
arachidonate + O2 | - |
Mus musculus | (5Z,8Z,10E,14Z)-(12S)-12-hydroperoxyicosa-5,8,10,14-tetraenoate | - |
? | |
arachidonate + O2 | - |
Mus musculus C57BL/6 | (5Z,8Z,10E,14Z)-(12S)-12-hydroperoxyicosa-5,8,10,14-tetraenoate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
12/15-lipoxygenase | - |
Mus musculus |
12/15-LOX | - |
Mus musculus |
Organism | Comment | Expression |
---|---|---|
Mus musculus | 12/15-LOX activity is enhanced during chronic, but not acute, toxoplasmosis | up |
General Information | Comment | Organism |
---|---|---|
physiological function | 12/15-LOX is a critical mediator of the chronic type 1 inflammatory response. Evolution of the immune response to Toxoplasma gondii is accompanied by an increasing requirement for 12/15-LOX mediated signaling. Although 12/15-LOX deficient mice are resistant to acute Toxoplasma gondii infection, 80% of 12/15-LOX-deficient mice die during chronic toxoplasmosis, compared to no deaths in wild-type controls. The enhanced susceptibility of 12/15-LOX-deficient mice to chronic toxoplasmosis is associated with reduced production of IL-12 and gamma interferon (IFN-gamma) that is not evident during acute infection. Ex vivo IFN-gamma production by 12/15-LOX-deficient splenocytes can be rescued by the addition of recombinant IL-12. 12/15-LOX does not play a role in macrophage killing of Toxoplasma gondii in vitro | Mus musculus |