Crystallization (Comment) | Organism |
---|---|
modeling of the protein dimer | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
F174A/W176A/L183E/L187E/Y191A | quintuple mutant disrupts both the hydrophobic and pi-pi interactions of the dimer interface, generating an 12-LOX monomer | Homo sapiens |
L183E/L187E | mutant mostly forms monomers, product ratio of 12S:15S-enantiomer is 78:22, strong decrease in inhibition by ML355 | Homo sapiens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
ML355 | - |
Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0026 | - |
arachidonate | wild-type, pH 8,23°C | Homo sapiens | |
0.011 | - |
arachidonate | mutant L183E/L187E, pH 8,23°C | Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | P18054 | - |
- |
Source Tissue | Comment | Organism | Textmining |
---|
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
arachidonate + O2 | - |
Homo sapiens | (5Z,8Z,10E,14Z)-(12S)-12-hydroperoxyeicosa-5,8,10,14-tetraenoate | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | modeling of the protein dimer shows a TOP-to-TOP dimeric arrangement with the alpha-helices containing a Leu-rich region (L172, L183, L187, and L194). Four nonoverlapping peptides within helix alpha2 of the TOP subdomain of wild-type are stabilizing, consistent with the dimer interface | Homo sapiens |
Synonyms | Comment | Organism |
---|---|---|
ALOX12 | - |
Homo sapiens |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
2.4 | - |
arachidonate | mutant L183E/L187E, pH 8,23°C | Homo sapiens | |
7 | - |
arachidonate | wild-type, pH 8,23°C | Homo sapiens |
IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
---|---|---|---|---|---|
0.00043 | - |
wild-type, pH 8,23°C | Homo sapiens | ML355 | |
0.1 | - |
above 0.1 mM, mutant L183E/L187E, pH 8,23°C | Homo sapiens | ML355 |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
200 | - |
arachidonate | mutant L183E/L187E, pH 8,23°C | Homo sapiens | |
2700 | - |
arachidonate | wild-type, pH 8,23°C | Homo sapiens |