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Literature summary for 1.13.11.31 extracted from

  • Goloshchapova, K.; Stehling, S.; Heydeck, D.; Blum, M.; Kuhn, H.
    Functional characterization of a novel arachidonic acid 12S-lipoxygenase in the halotolerant bacterium Myxococcus fulvus exhibiting complex social living patterns (2018), MicrobiologyOpen, 8, e775 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.13.11.31

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli and Sf9 cell Myxococcus fulvus

Protein Variants

Protein Variants Comment Organism
A410G 109% of wild-type activity, increase in enantioselectivity Myxococcus fulvus
F424I/I425M 75% of wild-type activity, 4fold increase in iron content Myxococcus fulvus
G359F catalytically inactive Myxococcus fulvus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.427
-
 5,8,11,14,17-eicosapentaenoic acid pH 7.4, temperature not specified in the publication Myxococcus fulvus

Localization

Localization Comment Organism GeneOntology No. Textmining
intracellular
-
 Myxococcus fulvus 5622
-

Metals/Ions

Metals/Ions Comment Organism Structure
Iron sequence contains functional iron ligand clusters C1 [His376 + His372] and C2 [His549 + Asn553], wild-type protein contains 5.6% iron load Myxococcus fulvus
additional information enzyme may not contain manganese Myxococcus fulvus

Organism

Organism UniProt Comment Textmining
Myxococcus fulvus
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4,7,10,13,16,19-docosahexaenoic acid + O2
-
 Myxococcus fulvus (14S)-14-hydroxy-4,7,10,13,16,19-docosahexaenoic acid
-
 ?
5,8,11,14,17-eicosapentaenoic acid + O2
-
 Myxococcus fulvus (12S)-12-hydroxy-5,8,10,14,17-eicosapentaenoic acid
-
 ?
alpha-linolenic acid + O2
-
 Myxococcus fulvus 13-hydroxy-(9Z,11E,15Z)-octadecatrienoic acid
-
 ?
arachidonate + O2
-
 Myxococcus fulvus (5Z,8Z,10E,14Z)-(12S)-12-hydroperoxyeicosa-5,8,10,14-tetraenoate
-
 ?
additional information no substrates: gamma-linolenic acid and linoleic acid Myxococcus fulvus ?
-
-

Subunits

Subunits Comment Organism
? x * 80000, SDS-PAGE, x * 79800, calculated from sequence, recombinant His-tagged protein Myxococcus fulvus

Synonyms

Synonyms Comment Organism
12S-lipoxygenase
-
 Myxococcus fulvus
LOX1
-
 Myxococcus fulvus
WP_046712474
-
 Myxococcus fulvus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.031
-
 5,8,11,14,17-eicosapentaenoic acid pH 7.4, temperature not specified in the publication Myxococcus fulvus

General Information

General Information Comment Organism
physiological function Lox1 converts arachidonic acid and other polyunsaturated fatty acids enantioselectively to the corresponding n-9 hydroperoxy derivatives. The enzyme prefers C20- and C22-polyenoic fatty acids but does not exhibit significant membrane oxygenase activity Myxococcus fulvus