Literature summary for 1.13.11.33 extracted from

Schurmann, K.; Anton, M.; Ivanov, I.; Richter, C.; Kuhn, H.; Walther, M.
Molecular basis for the reduced catalytic activity of the naturally occurring T560M mutant of human 12/15-lipoxygenase that has been implicated in coronary artery disease (2011), J. Biol. Chem., 286, 23920-23927.

Search for more literature for 1.13.11.33

Cloned(Commentary)

Cloned (Comment)	Organism
expressed in Escherichia coli as a His-tagged fusion protein	Homo sapiens

Protein Variants

Protein Variants	Comment	Organism
N287D	relative catalytic activity: 1.1% compared to wild-type 100%	Homo sapiens
N287L	relative catalytic activity: 0.5% compared to wild-type 100%	Homo sapiens
N287Q	relative catalytic activity: 3.9% compared to wild-type 100%	Homo sapiens
Q294L	relative catalytic activity: 0.6% compared to wild-type 100%	Homo sapiens
T560A	mutant shows strongly impaired catalytic activity, relative catalytic activity: 16.6% compared to wild-type 100%	Homo sapiens
T560M	mutant shows strongly impaired catalytic activity, relative catalytic activity: 3.8% compared to wild-type 100%	Homo sapiens
T560S	enzyme retains activity, relative catalytic activity: 70.7% compared to wild-type 100%	Homo sapiens

Organism

Organism	UniProt	Comment	Textmining
Homo sapiens	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
using Ni-NTA chromatography	Homo sapiens

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
arachidonic acid + O2	-	Homo sapiens	?	-	?

Synonyms

Synonyms	Comment	Organism
Alox15	-	Homo sapiens

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
25	-	assay at	Homo sapiens

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Release 2023.1 (January 2023)