Literature summary for 1.13.11.34 extracted from

Esser, J.; Rakonjac, M.; Hofmann, B.; Fischer, L.; Provost, P.; Schneider, G.; Steinhilber, D.; Samuelsson, B.; Radmark, O.
Coactosin-like protein functions as a stabilizing chaperone for 5-lipoxygenase: Role of tryptophan 102 (2010), Biochem. J., 425, 265-274.

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Activating Compound

Activating Compound	Comment	Organism	Structure
coactosin-like protein	binds 5-LO and promotes leukotriene formation, coactosin-like protein prevents heat-inactivation of 5-LO	Homo sapiens
phosphatidylcholine	-	Homo sapiens

Cloned(Commentary)

Cloned (Comment)	Organism
expressed in Escherichia coli BL21 and MV1190 cells	Homo sapiens

Protein Variants

Protein Variants	Comment	Organism
W102A	the mutation prevents interaction with coactosin-like protein, the mutant shows barely detectable dioxygenase activity	Homo sapiens
W13A	the mutation upregulates leukotriene production, the enzyme activity of the mutant 5-LO at low concentrations of phosphatidylcholine (0.005 mg/ml) and arachidonic acid (0.02 mM) is reduced compared to the wild type enzyme	Homo sapiens
W75A	the mutation upregulates leukotriene production, the enzyme activity of the mutant 5-LO at low concentrations of phosphatidylcholine (0.005 mg/ml) and arachidonic acid (0.02 mM) is reduced compared to the wild type enzyme	Homo sapiens

General Stability

General Stability	Organism
coactosin-like protein functions as a stabilizing chaperone for 5-lipoxygenase (preventing non-turnover inactivation)	Homo sapiens

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
nucleus	5-LO associates with a nuclear fraction only when differentiated cells are primed with phorbol ester and stimulated with ionophore	Homo sapiens	5634	-

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Ca2+	activates	Homo sapiens

Organism

Organism	UniProt	Comment	Textmining
Homo sapiens	-	-	-

Source Tissue

Source Tissue	Comment	Organism	Textmining
Mono-Mac-6 cell	-	Homo sapiens	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
(6E,8Z,11Z,14Z)-(5S)-5-hydroperoxyeicosa-6,8,11,14-tetraenoate	-	Homo sapiens	leukotriene A4 + H2O	-	?
arachidonate + O2	-	Homo sapiens	(6E,8Z,11Z,14Z)-(5S)-hydroperoxyicosa-6,8,11,14-tetraenoate	-	?

Synonyms

Synonyms	Comment	Organism
5-lipoxygenase	-	Homo sapiens
5-LO	-	Homo sapiens

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Release 2023.1 (January 2023)