Application | Comment | Organism |
---|---|---|
medicine | the enzyme is a promising target for anti-inflammatory therapy | Homo sapiens |
Cloned (Comment) | Organism |
---|---|
recombinant expression of His-tagged wild-type enzyme, enzyme mutant, and isolated enzyme PLAT domain in Escherichia coli strain BL21(DE3) | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
additional information | the isolated PLAT W75G interacts with calcium and the Dicer fragment | Homo sapiens |
W75G | site-directed mutagenesis, the mutation causes reduced aggregation and substantially increased thermal stability of the mutant. The mutant still shows Ca2+ interactions. Mutation W75G thermally stabilises the isolated PLAT domain | Homo sapiens |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytosol | - |
Homo sapiens | 5829 | - |
additional information | membrane-binding residue tryptophan 75 | Homo sapiens | - |
- |
nuclear membrane | - |
Homo sapiens | 31965 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | allosteric modulator involved in membrane binding by the enzyme | Homo sapiens |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
13800 | - |
isolated enzyme PLAT domain, gel filtration | Homo sapiens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
arachidonate + O2 | Homo sapiens | - |
(5S,6S,7E,9E,11Z,14Z)-5,6-epoxyicosa-7,9,11,14-tetraenoate + H2O | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | P09917 | - |
- |
Purification (Comment) | Organism |
---|---|
recombinant His-tagged wild-type enzyme and enzyme mutant from Escherichia coli strain BL21(DE3) by nickel affinity chromatography, tag cleavage by TEV protease, and gel filtration, isolated enzyme PLAT domain | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
arachidonate + O2 | - |
Homo sapiens | (5S,6S,7E,9E,11Z,14Z)-5,6-epoxyicosa-7,9,11,14-tetraenoate + H2O | - |
? |
Synonyms | Comment | Organism |
---|---|---|
5-lipoxygenase | - |
Homo sapiens |
5-LOX | - |
Homo sapiens |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Homo sapiens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.4 | - |
assay at | Homo sapiens |
General Information | Comment | Organism |
---|---|---|
additional information | the ability of the isolated PLAT domain to bind Dicer C-terminus whereas the interaction with coactosin-like protein requires the interplay of the catalytic and the PLAT domain | Homo sapiens |
physiological function | 5-lipoxygenase (5-LOX) is the key player of pro-inflammatory leukotriene biosynthesis. Its regulatory or socalled PLAT (polycystin-1, lipoxygenase, alpha-toxin) domain binds allosteric modulators like calcium, membranes, coactosin-like protein and Dicer, thereby influencing 5-LOX activity at the nuclearmembrane by mediating translocation. The PLAT domain may also regulate cytosolic 5-LOX activity and possibly influence microRNA metabolism | Homo sapiens |