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Literature summary for 1.13.11.36 extracted from

  • Muller, R.; Haug, S.; Eberspńcher, J.; Lingens, F.
    Catechol 2,3-dioxygenase from pyrazon-degrading bacteria (1977), Hoppe-Seyler's Z. Physiol. Chem., 358, 797-805.
    View publication on PubMed

General Stability

General Stability Organism
high osmotic sensitivity Phenylobacterium immobile

Inhibitors

Inhibitors Comment Organism Structure
high ionic strength
-
Phenylobacterium immobile

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.017
-
3-Methylpyrocatechol
-
Phenylobacterium immobile
0.16
-
4-Methylpyrocatechol
-
Phenylobacterium immobile
0.2
-
5-Amino-4-chloro-2-(2,3-dihydroxyphenyl)-3(2H)-pyridazinone
-
Phenylobacterium immobile
0.66
-
pyrocatechol
-
Phenylobacterium immobile

Metals/Ions

Metals/Ions Comment Organism Structure
Co2+ can partially replace Fe2+ Phenylobacterium immobile
Fe2+ required for activity Phenylobacterium immobile
Zn2+ can partially replace Fe2+ Phenylobacterium immobile

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
34000
-
6 * 34000, SDS-PAGE Phenylobacterium immobile
200000
-
analytical ultracentrifugation Phenylobacterium immobile
220000
-
gel filtration Phenylobacterium immobile

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
5-amino-4-chloro-2-(2,3-dihydroxyphenyl)-3(2H)-pyridazinone + O2 Phenylobacterium immobile enzyme of pyrazone metabolism 5-amino-4-chloro-2-(2-hydroxymuconoyl)-3(2H)-pyridazinone
-
?

Organism

Organism UniProt Comment Textmining
Phenylobacterium immobile
-
DSM 1986
-

Purification (Commentary)

Purification (Comment) Organism
-
Phenylobacterium immobile

Storage Stability

Storage Stability Organism
-20┬░C, stable for months Phenylobacterium immobile

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3-methylpyrocatechol + O2
-
Phenylobacterium immobile 2-hydroxy-3-methylmuconic acid semialdehyde
-
?
4-methylpyrocatechol + O2
-
Phenylobacterium immobile 2-hydroxy-3-methylmuconic acid semialdehyde
-
?
5-amino-4-chloro-2-(2,3-dihydroxyphenyl)-3(2H)-pyridazinone + O2
-
Phenylobacterium immobile 5-amino-4-chloro-2-(2-hydroxymuconoyl)-3(2H)-pyridazinone
-
?
5-amino-4-chloro-2-(2,3-dihydroxyphenyl)-3(2H)-pyridazinone + O2 enzyme of pyrazone metabolism Phenylobacterium immobile 5-amino-4-chloro-2-(2-hydroxymuconoyl)-3(2H)-pyridazinone
-
?
catechol + O2
-
Phenylobacterium immobile 2-hydroxymuconic acid semialdehyde
-
?

Subunits

Subunits Comment Organism
hexamer 6 * 34000, SDS-PAGE Phenylobacterium immobile

Temperature Optimum [┬░C]

Temperature Optimum [┬░C] Temperature Optimum Maximum [┬░C] Comment Organism
30
-
-
Phenylobacterium immobile

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7 8
-
Phenylobacterium immobile