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Literature summary for 1.13.11.37 extracted from

  • Ferraroni, M.; Seifert, J.; Travkin, V.M.; Thiel, M.; Kaschabek, S.; Scozzafava, A.; Golovleva, L.; Schlomann, M.; Briganti, F.
    Crystal structure of the hydroxyquinol 1,2-dioxygenase from Nocardioides simplex 3E, a key enzyme involved in polychlorinated aromatics biodegradation (2005), J. Biol. Chem., 280, 21144-21154.
    View publication on PubMed
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Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Pimelobacter simplex

Crystallization (Commentary)

Crystallization (Comment) Organism
sitting drop vapor difffusion from a solution containing 2 M ammonium sulfate, 4% polyethylene glycol 400, 100 mM Hepes, pH 7.5, drops consist of 0.004 ml of 20 mg/ml protein solution and 0.006 ml reservoir solution equilibrated against 0.05 ml of reservoir solution, crystals diffract to 1.75 A Pimelobacter simplex

Inhibitors

Inhibitors Comment Organism Structure
catechol
-
 Pimelobacter simplex

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0012
-
 Benzene-1,2,4-triol
-
 Pimelobacter simplex

Metals/Ions

Metals/Ions Comment Organism Structure
Fe3+ required for activity Pimelobacter simplex

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
benzene-1,2,4-triol + O2 Pimelobacter simplex
-
 3-hydroxy-cis,cis-muconate
-
 ?
benzene-1,2,4-triol + O2 Pimelobacter simplex 3E
-
 3-hydroxy-cis,cis-muconate
-
 ?

Organism

Organism UniProt Comment Textmining
Pimelobacter simplex Q5PXQ6 3E
-
Pimelobacter simplex 3E Q5PXQ6 3E
-

Purification (Commentary)

Purification (Comment) Organism
recombinant 1,2-HQD Pimelobacter simplex

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
benzene-1,2,4-triol + O2
-
 Pimelobacter simplex 3-hydroxy-cis,cis-muconate
-
 ?
benzene-1,2,4-triol + O2
-
 Pimelobacter simplex 3E 3-hydroxy-cis,cis-muconate
-
 ?

Synonyms

Synonyms Comment Organism
1,2-HQD
-
 Pimelobacter simplex

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
29
-
 Benzene-1,2,4-triol
-
 Pimelobacter simplex

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
10
-
 catechol approximate value Pimelobacter simplex