Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Nocardioides sp. |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
3-hydroxy-2-naphthoate | competitive | Nocardioides sp. | |
additional information | no inhibition when the concentration of the substrate 1-hydroxy-2-naphthoate is 0.01 mM and that of the tested compounds is 0.1 or 1 mM: gentisate, 3-hydroxyanthranilate, 2-hydroxy-1-naphthoate, salicylate, m-hydroxybenzoate, p-hydroxybenzoate, and protocatechuate | Nocardioides sp. | |
o-phenanthroline | 0.1 mM, reactivation by incubating with FeSO4 and ascorbic acid | Nocardioides sp. |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.01 | - |
1-hydroxy-2-naphthoate | pH 7.5, 25°C | Nocardioides sp. |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Fe2+ | enzyme contains 1.15 mol of Fe(II)/mol of the subunit. Fe2+ is required for the enzyme to exhibit activity | Nocardioides sp. |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
45000 | - |
6 * 45000, SDS-PAGE | Nocardioides sp. |
270000 | - |
gel filtration | Nocardioides sp. |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
1-hydroxy-2-naphthoate + O2 | Nocardioides sp. | the enzyme is involved in degradation of phenanthrene | (3E)-4-(2-carboxyphenyl)-2-oxobut-3-enoate | i.e. trans-2'-carboxybenzalpyruvate | ? | |
1-hydroxy-2-naphthoate + O2 | Nocardioides sp. KP7 | the enzyme is involved in degradation of phenanthrene | (3E)-4-(2-carboxyphenyl)-2-oxobut-3-enoate | i.e. trans-2'-carboxybenzalpyruvate | ? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Nocardioides sp. | O24721 | - |
- |
Nocardioides sp. KP7 | O24721 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Nocardioides sp. |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
culture condition:phenanthrene-grown cell | - |
Nocardioides sp. | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
8.4 | - |
enzyme in extract of strain KP7 | Nocardioides sp. |
180 | - |
purified enzyme | Nocardioides sp. |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
1-hydroxy-2-naphthoate + O2 | - |
Nocardioides sp. | (3E)-4-(2-carboxyphenyl)-2-oxobut-3-enoate | i.e. trans-2'-carboxybenzalpyruvate | ? | |
1-hydroxy-2-naphthoate + O2 | the enzyme is involved in degradation of phenanthrene | Nocardioides sp. | (3E)-4-(2-carboxyphenyl)-2-oxobut-3-enoate | i.e. trans-2'-carboxybenzalpyruvate | ? | |
1-hydroxy-2-naphthoate + O2 | - |
Nocardioides sp. KP7 | (3E)-4-(2-carboxyphenyl)-2-oxobut-3-enoate | i.e. trans-2'-carboxybenzalpyruvate | ? | |
1-hydroxy-2-naphthoate + O2 | the enzyme is involved in degradation of phenanthrene | Nocardioides sp. KP7 | (3E)-4-(2-carboxyphenyl)-2-oxobut-3-enoate | i.e. trans-2'-carboxybenzalpyruvate | ? | |
additional information | no activity with: gentisate, 3-hydroxyanthranilate, 2-hydroxy-1-naphthoate, 3-hydroxy-2-naphthoate, salicylate, m-hydroxybenzoate, p-hydroxybenzoate, and protocatechuate | Nocardioides sp. | ? | - |
? | |
additional information | no activity with: gentisate, 3-hydroxyanthranilate, 2-hydroxy-1-naphthoate, 3-hydroxy-2-naphthoate, salicylate, m-hydroxybenzoate, p-hydroxybenzoate, and protocatechuate | Nocardioides sp. KP7 | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
hexamer | 6 * 45000, SDS-PAGE | Nocardioides sp. |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | - |
- |
Nocardioides sp. |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
114 | - |
1-hydroxy-2-naphthoate | pH 7.5, 25°C | Nocardioides sp. |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
- |
Nocardioides sp. |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.022 | - |
3-hydroxy-2-naphthoate | pH 7.5, 25°C | Nocardioides sp. |