Cloned (Comment) | Organism |
---|---|
- |
Pseudomonas oleovorans |
bphC gene cloned | Pseudomonas aeruginosa |
nucleotide sequence of gene bphC cloned into pQE1 | Pseudomonas putida |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
1,10-phenanthroline | 5 mM 30 min | Pseudomonas putida | |
8-hydroxyquinoline | 5 mM 30 min | Pseudomonas putida | |
CdCl2 | - |
Pseudomonas putida | |
CuSO4 | - |
Pseudomonas putida | |
diethyldithiocarbamic acid | 5 mM 30 min | Pseudomonas putida | |
Fe3+ | - |
Pseudomonas putida | |
FeCl3 | - |
Pseudomonas putida | |
HgCl2 | - |
Pseudomonas putida | |
iodoacetic acid | 5 mM 30 min | Pseudomonas putida | |
N-ethylmaleimide | 5 mM 30 min | Pseudomonas putida | |
NiSO4 | - |
Pseudomonas putida |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0145 | - |
2,3-Dihydroxybiphenyl | strain OU83 | Pseudomonas putida |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Fe2+ | - |
Comamonas testosteroni | |
Fe2+ | significantly enhances activity | Pseudomonas putida |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
34000 | - |
SDS-PAGE | Pseudomonas putida |
270000 | - |
gel filtration | Pseudomonas putida |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
biphenyl-2,3-diol + O2 | Pseudomonas aeruginosa | - |
2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoate + H2O | - |
? | |
biphenyl-2,3-diol + O2 | Cupriavidus necator | - |
2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoate + H2O | - |
? | |
biphenyl-2,3-diol + O2 | Comamonas testosteroni | - |
2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoate + H2O | - |
? | |
biphenyl-2,3-diol + O2 | Acinetobacter sp. | - |
2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoate + H2O | - |
? | |
biphenyl-2,3-diol + O2 | Pseudomonas oleovorans | - |
2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoate + H2O | - |
? | |
biphenyl-2,3-diol + O2 | Sphingobium yanoikuyae | - |
2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoate + H2O | - |
? | |
biphenyl-2,3-diol + O2 | Pseudomonas putida | third step in biphenyl degradation pathway | 2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoate + H2O | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Acinetobacter sp. | - |
- |
- |
Comamonas testosteroni | - |
- |
- |
Cupriavidus necator | - |
- |
- |
Pseudomonas aeruginosa | - |
- |
- |
Pseudomonas oleovorans | - |
- |
- |
Pseudomonas putida | Q52032 | - |
- |
Sphingobium yanoikuyae | - |
formerly Pseudomonas paucimobilis strain Q1 | - |
Purification (Comment) | Organism |
---|---|
recombinant enzyme, expressed in Escherichia coli SG13009(pREP4)(pAKC1) | Pseudomonas putida |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-chloro-2,3-dihydroxybiphenyl + O2 | - |
Comamonas testosteroni | 3-chloro-2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoate | - |
? | |
4-chloro-2,3-dihydroxybiphenyl + O2 | - |
Pseudomonas putida | 3-chloro-2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoate | - |
? | |
4-chlorocatechol + O2 | - |
Pseudomonas oleovorans | 4-chloro-2-hydroxy-6-oxohexa-2,4-dienoate | - |
? | |
4-chlorocatechol + O2 | - |
Sphingobium yanoikuyae | 4-chloro-2-hydroxy-6-oxohexa-2,4-dienoate | - |
? | |
4-chlorocatechol + O2 | - |
Pseudomonas putida | 4-chloro-2-hydroxy-6-oxohexa-2,4-dienoate | - |
? | |
4-methylcatechol + O2 | - |
Pseudomonas oleovorans | 2-hydroxy-4-methyl-6-oxohexa-2,4-dienoate | - |
? | |
4-methylcatechol + O2 | - |
Sphingobium yanoikuyae | 2-hydroxy-4-methyl-6-oxohexa-2,4-dienoate | - |
? | |
4-methylcatechol + O2 | - |
Pseudomonas putida | 2-hydroxy-4-methyl-6-oxohexa-2,4-dienoate | - |
? | |
biphenyl-2,3-diol + O2 | - |
Pseudomonas aeruginosa | 2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoate + H2O | - |
? | |
biphenyl-2,3-diol + O2 | - |
Cupriavidus necator | 2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoate + H2O | - |
? | |
biphenyl-2,3-diol + O2 | - |
Comamonas testosteroni | 2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoate + H2O | - |
? | |
biphenyl-2,3-diol + O2 | - |
Acinetobacter sp. | 2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoate + H2O | - |
? | |
biphenyl-2,3-diol + O2 | - |
Pseudomonas oleovorans | 2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoate + H2O | - |
? | |
biphenyl-2,3-diol + O2 | - |
Sphingobium yanoikuyae | 2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoate + H2O | - |
? | |
biphenyl-2,3-diol + O2 | - |
Pseudomonas putida | 2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoate + H2O | - |
? | |
biphenyl-2,3-diol + O2 | third step in biphenyl degradation pathway | Pseudomonas putida | 2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoate + H2O | - |
? | |
catechol + O2 | - |
Pseudomonas oleovorans | ? | - |
? | |
catechol + O2 | - |
Sphingobium yanoikuyae | ? | - |
? | |
catechol + O2 | - |
Pseudomonas putida | ? | - |
? | |
additional information | - |
Sphingobium yanoikuyae | ? | - |
? | |
additional information | strain 3,4-dihydroxybiphenyl is no substrate | Comamonas testosteroni | ? | - |
? | |
additional information | strain 3,4-dihydroxybiphenyl is no substrate | Pseudomonas putida | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
octamer | - |
Pseudomonas oleovorans |
octamer | 8 * 34000, SDS-PAGE | Pseudomonas putida |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
- |
Pseudomonas putida |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | 65 | loses 47% of the activity at 37°C and all activity at 65°C | Pseudomonas putida |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
- |
Pseudomonas putida |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
7 | 9 | - |
Pseudomonas putida |