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Literature summary for 1.13.11.39 extracted from

  • Furukawa, K.; Arimura, N.
    Purification and properties of 2,3-dihydroxybiphenyl dioxygenase from polychlorinated biphenyl-degrading Pseudomonas pseudoalcaligenes and Pseudomonas aeruginosa carrying the cloned bphC gene (1987), J. Bacteriol., 169, 924-927.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.13.11.39

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Pseudomonas aeruginosa Pseudomonas oleovorans

Inhibitors

Inhibitors Comment Organism Structure
Fe3+ complete inactivation Pseudomonas oleovorans

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.087
-
 biphenyl-2,3-diol pH 9.0, 25°C Pseudomonas oleovorans

Metals/Ions

Metals/Ions Comment Organism Structure
Fe2+ required, after loss of ferrous iron, activity can be restored by incubation with ferrous iron in presence of cysteine Pseudomonas oleovorans

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
31000
-
 8 * 31000, SDS-PAGE Pseudomonas oleovorans
250000
-
 PAGE Pseudomonas oleovorans

Organism

Organism UniProt Comment Textmining
Pseudomonas oleovorans
-
 native enzyme and expressed in Pseudomonas aeruginosa
-

Renatured (Commentary)

Renatured (Comment) Organism
after loss of ferrous iron, activity can be restored by incubation with ferrous iron in presence of cysteine Pseudomonas oleovorans

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
87.2
-
 pH 9.0, 25°C Pseudomonas oleovorans

Storage Stability

Storage Stability Organism
stable after addition of 10% acetone or 10% ethanol Pseudomonas oleovorans

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
biphenyl-2,3-diol + O2
-
 Pseudomonas oleovorans 2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoate + H2O
-
 ?
additional information no substrate: biphenyl-3,4-diol, catechol, 3-methylcatechol, 4-methylcatechol Pseudomonas oleovorans ?
-
 ?

Subunits

Subunits Comment Organism
More N-terminal amino acid sequence Pseudomonas oleovorans
octamer 8 * 31000, SDS-PAGE Pseudomonas oleovorans

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
9
-
-
 Pseudomonas oleovorans

pI Value

Organism Comment pI Value Maximum pI Value
Pseudomonas oleovorans isoelectric focusing
-
 4.5