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Literature summary for 1.13.11.39 extracted from

  • Ishida, T.; Senda, T.; Tanaka, H.; Yamamoto, A.; Horiike, K.
    Single-turnover kinetics of 2,3-dihydroxybiphenyl 1,2-dioxygenase reacting with 3-formylcatechol (2005), Biochem. Biophys. Res. Commun., 338, 223-229.
    View publication on PubMed
Search for more literature for 1.13.11.39

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3)pLysS cells Pseudomonas sp.

Inhibitors

Inhibitors Comment Organism Structure
2-Hydroxypyridine N-oxide 0.2 mM Pseudomonas sp.

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.00046
-
 biphenyl-2,3-diol in 50 mM Hepes (pH 7.5) at 25°C Pseudomonas sp.
0.0108
-
 3-formylcatechol in 50 mM Hepes (pH 7.5) at 25°C Pseudomonas sp.
0.0589
-
 catechol in 50 mM Hepes (pH 7.5) at 25°C Pseudomonas sp.

Organism

Organism UniProt Comment Textmining
Pseudomonas sp.
-
-
-

Purification (Commentary)

Purification (Comment) Organism
Sephadex G-25 column gel filtration Pseudomonas sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3-formylcatechol + O2
-
 Pseudomonas sp. ?
-
 ?
biphenyl-2,3-diol + O2
-
 Pseudomonas sp. 2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoate + H2O
-
 ?
catechol + O2
-
 Pseudomonas sp. ?
-
 ?

Synonyms

Synonyms Comment Organism
2,3-dihydroxybiphenyl 1,2-dioxygenase
-
 Pseudomonas sp.
BphC
-
 Pseudomonas sp.

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.8
-
 3-formylcatechol in 50 mM Hepes (pH 7.5) at 25°C Pseudomonas sp.
32.5
-
 catechol in 50 mM Hepes (pH 7.5) at 25°C Pseudomonas sp.
115
-
 biphenyl-2,3-diol in 50 mM Hepes (pH 7.5) at 25°C Pseudomonas sp.