Cloned (Comment) | Organism |
---|---|
CarBaBb gene from Pseudomonas resinovorans strain CA10 expressed by expression vector pUCA503 in Escherichia coli BL21(DE3) | Pseudomonas resinovorans |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.014 | - |
2'-aminobiphenyl-2,3-diol | pH 7.5, 25°C | Pseudomonas resinovorans |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
10000 | - |
alpha2beta2, 2 * 10000 CarBa, 2 * 29000 CarBb, determination by SDS-PAGE | Pseudomonas resinovorans |
29000 | - |
alpha2beta2, 2 * 10000 CarBa, 2 * 29000 CarBb, determination by SDS-PAGE | Pseudomonas resinovorans |
78000 | - |
heterotetramer, gel-filtration chromatography | Pseudomonas resinovorans |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas resinovorans | - |
- |
- |
Pseudomonas resinovorans CA10 | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2'-aminobiphenyl-2,3-diol + O2 | - |
Pseudomonas resinovorans | (2E,4E)-6-(2-aminophenyl)-2-hydroxy-6-oxohexa-2,4-dienoate + H+ | - |
? | |
2'-aminobiphenyl-2,3-diol + O2 | - |
Pseudomonas resinovorans CA10 | (2E,4E)-6-(2-aminophenyl)-2-hydroxy-6-oxohexa-2,4-dienoate + H+ | - |
? |
Subunits | Comment | Organism |
---|---|---|
heterotetramer | alpha2beta2, 2 * 10000 CarBa, 2 * 29000 CarBb, determination by SDS-PAGE | Pseudomonas resinovorans |
Synonyms | Comment | Organism |
---|---|---|
2'-aminobiphenyl-2,3-diol 1,2-dioxygenase | - |
Pseudomonas resinovorans |
CarB | - |
Pseudomonas resinovorans |
CarBaBb | - |
Pseudomonas resinovorans |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
35 | - |
- |
Pseudomonas resinovorans |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
30 min full activity | Pseudomonas resinovorans |
60 | - |
30 min, enzyme retains 30% activity | Pseudomonas resinovorans |
70 | - |
30 min, enzyme completely inactivated | Pseudomonas resinovorans |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.5 | - |
- |
Pseudomonas resinovorans |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
7 | 9 | - |
Pseudomonas resinovorans |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
250 | - |
2'-aminobiphenyl-2,3-diol | pH 7.5, 25°C | Pseudomonas resinovorans |