Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 1.13.11.39 extracted from

  • Shi, S.; Ma, F.; Sun, T.; Li, A.; Zhou, J.; Qu, Y.
    Multistep conversion of cresols by phenol hydroxylase and 2,3-dihydroxy-biphenyl 1,2-dioxygenase (2014), Front. Environ. Sci. Eng., 8, 539-546 .
No PubMed abstract available

Application

Application Comment Organism
synthesis a multistep conversion system composed of Pseudomonas sp. phenol hydroxylase (PHIND) and Burkholderia xenovorans 2,3-dihydroxy-biphenyl 1,2-dioxygenase (BphCLA-4) from is used to synthesize methylcatechols and semialdehydes from o- and m-cresol Paraburkholderia xenovorans

Cloned(Commentary)

Cloned (Comment) Organism
gene bphC, recombinant expression in Escherichia coli Paraburkholderia xenovorans

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
biphenyl-2,3-diol + O2 Paraburkholderia xenovorans
-
2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoate
-
?

Organism

Organism UniProt Comment Textmining
Paraburkholderia xenovorans
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3-methylcatechol + O2
-
Paraburkholderia xenovorans 2-hydroxy-6-oxohepta-2,4-dienoate
-
?
4-methylcatechol + O2 low activity Paraburkholderia xenovorans 2-hydroxy-4-methyl-6-oxohexa-2,4-dienoate
-
?
biphenyl-2,3-diol + O2
-
Paraburkholderia xenovorans 2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoate
-
?
additional information multistep conversion system composed of Pseudomonas sp. phenol hydroxylase (PHIND) and Burkholderia xenovorans 2,3-dihydroxy-biphenyl 1,2-dioxygenase (BphCLA-4) from is used to synthesize methylcatechols and semialdehydes from o- and m-cresol, substrate specificity and molecular docking, product identification by HPLC-MS, overview Paraburkholderia xenovorans ?
-
?

Synonyms

Synonyms Comment Organism
2,3-dihydroxy-biphenyl 1,2-dioxygenase
-
Paraburkholderia xenovorans
BphCLA-4
-
Paraburkholderia xenovorans

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
-
Paraburkholderia xenovorans

pH Range

pH Minimum pH Maximum Comment Organism
5 10 over 60% at pH 6.0 and 9.0, and less than 50% catalytic efficiency at pH 5.0 and 10.0, strain BphCLA-4 Paraburkholderia xenovorans

General Information

General Information Comment Organism
additional information enzyme structure homology modeling, overview Paraburkholderia xenovorans