Activating Compound | Comment | Organism | Structure |
---|---|---|---|
ascorbate | - |
Comamonas testosteroni | |
ascorbate | - |
Delftia acidovorans | |
dithiothreitol | - |
Comamonas testosteroni | |
dithiothreitol | - |
Delftia acidovorans |
General Stability | Organism |
---|---|
cysteine stabilizes | Comamonas testosteroni |
cysteine stabilizes | Delftia acidovorans |
Fe2+ stabilizes | Comamonas testosteroni |
Fe2+ stabilizes | Delftia acidovorans |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.015 | - |
4-Fluorogentisate | - |
Delftia acidovorans | |
0.018 | - |
3-Bromogentisate | - |
Delftia acidovorans | |
0.025 | - |
4-Chlorogentisate | - |
Delftia acidovorans | |
0.027 | - |
3-Methylgentisate | - |
Delftia acidovorans | |
0.035 | - |
3-Fluorogentisate | - |
Delftia acidovorans | |
0.044 | - |
4-Fluorogentisate | - |
Comamonas testosteroni | |
0.045 | - |
1,4-dihydroxy-2-naphthoate | - |
Delftia acidovorans | |
0.048 | - |
3-Isopropylgentisate | - |
Delftia acidovorans | |
0.05 | - |
3-Fluorogentisate | - |
Comamonas testosteroni | |
0.057 | - |
4-Methylgentisate | - |
Delftia acidovorans | |
0.074 | - |
gentisate | - |
Delftia acidovorans | |
0.085 | - |
gentisate | - |
Comamonas testosteroni | |
0.096 | - |
O2 | - |
Comamonas testosteroni | |
0.14 | - |
3,4-Dimethylgentisate | - |
Delftia acidovorans | |
0.24 | - |
4-Chlorogentisate | - |
Comamonas testosteroni | |
0.29 | - |
1,4-dihydroxy-2-naphthoate | - |
Comamonas testosteroni | |
0.33 | - |
3-Bromogentisate | - |
Comamonas testosteroni | |
0.45 | - |
4-O-Methylgentisate | - |
Comamonas testosteroni | |
0.52 | - |
4-Methylgentisate | - |
Comamonas testosteroni | |
1.18 | - |
3-O-Methylgentisate | - |
Comamonas testosteroni | |
1.2 | - |
4-Isopropylgentisate | - |
Comamonas testosteroni | |
1.23 | - |
4-Isopropylgentisate | - |
Comamonas testosteroni | |
1.75 | - |
3-Methylgentisate | - |
Comamonas testosteroni | |
2.75 | - |
3-Isopropylgentisate | - |
Comamonas testosteroni | |
3.65 | - |
3-Ethylgentisate | - |
Comamonas testosteroni | |
7.7 | - |
3,4-Dimethylgentisate | - |
Comamonas testosteroni |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Iron | 1.9-3.9 mol Fe2+ per subunit | Comamonas testosteroni | |
Iron | 1.3-3.2 mol Fe2+ per subunit | Delftia acidovorans | |
Iron | (alphaFe)4 structure | Comamonas testosteroni | |
Iron | (alphaFe)4 structure | Delftia acidovorans |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
40800 | - |
4 * 40800, SDS-PAGE | Comamonas testosteroni |
158000 | - |
gel filtration | Comamonas testosteroni |
164000 | - |
gel filtration | Delftia acidovorans |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Comamonas testosteroni | - |
- |
- |
Delftia acidovorans | - |
- |
- |
Oxidation Stability | Organism |
---|---|
exposure of enzyme to oxidants, e.g. H2O2 or K3Fe(CN)6, at 1 mM concentrations results in complete loss of activity, even under anaerobic conditions | Comamonas testosteroni |
exposure of enzyme to oxidants, e.g. H2O2 or K3Fe(CN)6, at 1 mM concentrations results in complete loss of activity, even under anaerobic conditions | Delftia acidovorans |
Purification (Comment) | Organism |
---|---|
- |
Comamonas testosteroni |
- |
Delftia acidovorans |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
484 | - |
- |
Delftia acidovorans |
498 | - |
- |
Comamonas testosteroni |
Storage Stability | Organism |
---|---|
-80°C, storage following rapid freezing in liquid N2, protein concentration above 10 mg/ml, about 15% loss of activity after 1 year | Comamonas testosteroni |
-80°C, storage following rapid freezing in liquid N2, protein concentration above 10 mg/ml, about 15% loss of activity after 1 year | Delftia acidovorans |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
1,4-dihydroxy-2-naphthoate + O2 | - |
Comamonas testosteroni | ? | - |
? | |
1,4-dihydroxy-2-naphthoate + O2 | - |
Delftia acidovorans | ? | - |
? | |
2,5-dihydroxybenzoate + O2 | - |
Comamonas testosteroni | maleylpyruvate | - |
? | |
2,5-dihydroxybenzoate + O2 | - |
Delftia acidovorans | maleylpyruvate | - |
? | |
3,4-dimethylgentisate + O2 | - |
Comamonas testosteroni | ? | - |
? | |
3,4-dimethylgentisate + O2 | - |
Delftia acidovorans | ? | - |
? | |
3-bromogentisate + O2 | - |
Comamonas testosteroni | 3-bromo-4-hydroxy-6-oxo-hepta-2,4-dienedioic acid | - |
? | |
3-bromogentisate + O2 | - |
Delftia acidovorans | 3-bromo-4-hydroxy-6-oxo-hepta-2,4-dienedioic acid | - |
? | |
3-ethylgentisate + O2 | - |
Comamonas testosteroni | 3-ethyl-4-hydroxy-6-oxo-hepta-2,4-dienedioic acid | - |
? | |
3-ethylgentisate + O2 | - |
Delftia acidovorans | 3-ethyl-4-hydroxy-6-oxo-hepta-2,4-dienedioic acid | - |
? | |
3-fluorogentisate + O2 | - |
Comamonas testosteroni | 3-fluoro-4-hydroxy-6-oxo-hepta-2,4-dienedioic acid | - |
? | |
3-fluorogentisate + O2 | - |
Delftia acidovorans | 3-fluoro-4-hydroxy-6-oxo-hepta-2,4-dienedioic acid | - |
? | |
3-hydroxybenzoate + O2 | minor pathway | Comamonas testosteroni | 3,4-dihydroxy-6-oxo-hepta-2,4-dienedioic acid | - |
? | |
3-hydroxybenzoate + O2 | main pathway | Delftia acidovorans | 3,4-dihydroxy-6-oxo-hepta-2,4-dienedioic acid | - |
? | |
3-isopropylgentisate + O2 | - |
Comamonas testosteroni | 4-hydroxy-3-isopropyl-6-oxo-hepta-2,4-dienedioic acid | - |
? | |
3-isopropylgentisate + O2 | - |
Delftia acidovorans | 4-hydroxy-3-isopropyl-6-oxo-hepta-2,4-dienedioic acid | - |
? | |
3-methylgentisate + O2 | - |
Comamonas testosteroni | 4-hydroxy-3-methyl-6-oxo-hepta-2,4-dienedioic acid | - |
? | |
3-methylgentisate + O2 | - |
Delftia acidovorans | 4-hydroxy-3-methyl-6-oxo-hepta-2,4-dienedioic acid | - |
? | |
3-O-methylgentisate + O2 | - |
Comamonas testosteroni | ? | - |
? | |
3-O-methylgentisate + O2 | - |
Delftia acidovorans | ? | - |
? | |
4-chlorogentisate + O2 | - |
Comamonas testosteroni | 2-chloro-4-hydroxy-6-oxo-hepta-2,4-dienedioic acid | - |
? | |
4-chlorogentisate + O2 | - |
Delftia acidovorans | 2-chloro-4-hydroxy-6-oxo-hepta-2,4-dienedioic acid | - |
? | |
4-fluorogentisate + O2 | - |
Comamonas testosteroni | 2-fluoro-4-hydroxy-6-oxo-hepta-2,4-dienedioic acid | - |
? | |
4-fluorogentisate + O2 | - |
Delftia acidovorans | 2-fluoro-4-hydroxy-6-oxo-hepta-2,4-dienedioic acid | - |
? | |
4-isopropylgentisate + O2 | - |
Comamonas testosteroni | 4-hydroxy-2-isopropyl-6-oxo-hepta-2,4-dienedioic acid | - |
? | |
4-isopropylgentisate + O2 | - |
Delftia acidovorans | 4-hydroxy-2-isopropyl-6-oxo-hepta-2,4-dienedioic acid | - |
? | |
4-methylgentisate + O2 | - |
Comamonas testosteroni | 4-hydroxy-2-methyl-6-oxo-hepta-2,4-dienedioic acid | - |
? | |
4-methylgentisate + O2 | - |
Delftia acidovorans | 4-hydroxy-2-methyl-6-oxo-hepta-2,4-dienedioic acid | - |
? | |
4-O-methylgentisate + O2 | - |
Comamonas testosteroni | ? | - |
? | |
4-O-methylgentisate + O2 | - |
Delftia acidovorans | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
tetramer | 4 * 37200-39800, SDS-PAGE | Delftia acidovorans |
tetramer | 4 * 40800, SDS-PAGE | Comamonas testosteroni |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
322 | 328 | gentisate | - |
Delftia acidovorans | |
367 | 642 | gentisate | - |
Comamonas testosteroni |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | 9 | - |
Comamonas testosteroni |
7 | 9 | - |
Delftia acidovorans |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
6 | 8 | unstable below and above | Comamonas testosteroni |
6 | 8 | unstable below and above | Delftia acidovorans |
7.4 | - |
highest stability | Comamonas testosteroni |
7.4 | - |
highest stability | Delftia acidovorans |