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Literature summary for 1.13.11.4 extracted from

  • Shen, X.H.; Jiang, C.Y.; Huang, Y.; Liu, Z.P.; Liu, S.J.
    Functional identification of novel genes involved in the glutathione-independent gentisate pathway in Corynebacterium glutamicum (2005), Appl. Environ. Microbiol., 71, 3442-3452.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.13.11.4

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli strain BL21/pET28ancg12920 Corynebacterium glutamicum

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
44000
-
 4 * 44000, gel filtration Corynebacterium glutamicum
180000
-
 SDS-PAGE Corynebacterium glutamicum

Organism

Organism UniProt Comment Textmining
Corynebacterium glutamicum
-
-
-
Corynebacterium glutamicum RES167
-
-
-

Purification (Commentary)

Purification (Comment) Organism
His-Bind protein chromatography Corynebacterium glutamicum

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
 the protein shows gentisate 1,2-dioxygenase specific activity of 0.17 U/mg of protein Corynebacterium glutamicum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2,5-dihydroxybenzoate + O2
-
 Corynebacterium glutamicum maleylpyruvate
-
 ?
2,5-dihydroxybenzoate + O2
-
 Corynebacterium glutamicum RES167 maleylpyruvate
-
 ?
3-hydroxybenzoate + O2
-
 Corynebacterium glutamicum 3,4-dihydroxy-6-oxo-hepta-2,4-dienedioic acid
-
 ?
3-hydroxybenzoate + O2
-
 Corynebacterium glutamicum RES167 3,4-dihydroxy-6-oxo-hepta-2,4-dienedioic acid
-
 ?
gentisate + O2
-
 Corynebacterium glutamicum ?
-
 ?
gentisate + O2
-
 Corynebacterium glutamicum RES167 ?
-
 ?

Subunits

Subunits Comment Organism
homotetramer 4 * 44000, gel filtration Corynebacterium glutamicum

Synonyms

Synonyms Comment Organism
gentisate dioxygenase
-
 Corynebacterium glutamicum