Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 1.13.11.4 extracted from

  • Subbotina, N.M.; Chernykh, A.M.; Taranov, A.I.; Shebanova, A.D.; Moiseeva, O.V.; Ferraroni, M.; Kolomytseva, M.P.
    Gentisate 1,2-dioxygenase from the gram-positive bacteria Rhodococcus opacus 1CP Identical active sites vs. different substrate selectivities (2021), Biochimie, 180, 90-103 .
    View publication on PubMed
Search for more literature for 1.13.11.4

Application

Application Comment Organism
analysis high potential for use in biosensor technologies for monitoring the metabolism of tyrosine and salicylic acid, as well as a pollution of environment and different materials by polyaromatic compounds Rhodococcus opacus

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli strain BL21 (DE3) Rhodococcus opacus

Inhibitors

Inhibitors Comment Organism Structure
2,3-Dihydroxybenzoate
-
 Rhodococcus opacus
2,4-Dihydroxybenzoate
-
 Rhodococcus opacus
2,6-dihydroxybenzoate
-
 Rhodococcus opacus
4-Chlorocatechol
-
 Rhodococcus opacus
4-methylcatechol
-
 Rhodococcus opacus
5-chlorosalicylate
-
 Rhodococcus opacus
catechol
-
 Rhodococcus opacus
homogentisate
-
 Rhodococcus opacus
Hydroxyquinol
-
 Rhodococcus opacus
salicylate
-
 Rhodococcus opacus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.04
-
 2,5-Dihydroxybenzoate pH 8.5, 25°C Rhodococcus opacus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
39600
-
 calculated from sequence Rhodococcus opacus
177000
-
 gel filtration Rhodococcus opacus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2,5-dihydroxybenzoate + O2 Rhodococcus opacus
-
 maleylpyruvate
-
 ?
2,5-dihydroxybenzoate + O2 Rhodococcus opacus 1CP
-
 maleylpyruvate
-
 ?

Organism

Organism UniProt Comment Textmining
Rhodococcus opacus W5U174
-
-
Rhodococcus opacus 1CP W5U174
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Rhodococcus opacus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2,5-dihydroxybenzoate + O2
-
 Rhodococcus opacus maleylpyruvate
-
 ?
2,5-dihydroxybenzoate + O2 narrow substrate specificity Rhodococcus opacus maleylpyruvate
-
 ?
2,5-dihydroxybenzoate + O2
-
 Rhodococcus opacus 1CP maleylpyruvate
-
 ?
2,5-dihydroxybenzoate + O2 narrow substrate specificity Rhodococcus opacus 1CP maleylpyruvate
-
 ?

Subunits

Subunits Comment Organism
tetramer 4 * 41000, SDS-PAGE Rhodococcus opacus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
40 45
-
 Rhodococcus opacus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.6 8 in 50 mM Tris/H2SO4 buffer Rhodococcus opacus
8
-
 in 50 mM Clark and Lubs phosphate buffer Rhodococcus opacus
8 8.3 in 50 mM Tris/HCl buffer Rhodococcus opacus

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.00145
-
 4-Chlorocatechol pH 8.5, 25°C Rhodococcus opacus
0.0067
-
 4-methylcatechol pH 8.5, 25°C Rhodococcus opacus
0.0449
-
 catechol pH 8.5, 25°C Rhodococcus opacus
0.057
-
 5-chlorosalicylate pH 8.5, 25°C Rhodococcus opacus
0.22
-
 2,6-dihydroxybenzoate pH 8.5, 25°C Rhodococcus opacus
0.33
-
 Hydroxyquinol pH 8.5, 25°C Rhodococcus opacus
0.444
-
 homogentisate pH 8.5, 25°C Rhodococcus opacus
0.81
-
 salicylate pH 8.5, 25°C Rhodococcus opacus
1.069
-
 2,3-Dihydroxybenzoate pH 8.5, 25°C Rhodococcus opacus
1.13
-
 2,4-Dihydroxybenzoate pH 8.5, 25°C Rhodococcus opacus