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Literature summary for 1.13.11.40 extracted from

  • Brash, A.R.; Boeglin, W.E.; Chang, M.S.; Shieh, B.H.
    Purification and molecular cloning of an 8R-lipoxygenase from the coral Plexaura homomalla reveal the related primary structures of R- and S-lipoxygenases (1996), J. Biol. Chem., 271, 20949-20957.
    View publication on PubMed
Search for more literature for 1.13.11.40

Cloned(Commentary)

Cloned (Comment) Organism
full-length cDNA cloned by PCR Plexaura homomalla

General Stability

General Stability Organism
unstable in solution, activity is completely lost after standing on ice overnight Plexaura homomalla

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
76000
-
 mature protein predicted from the cDNA, difference from the size estimated by SDS-PAGE implies a post-translational modification of the enzyme Plexaura homomalla
100000
-
 SDS-PAGE Plexaura homomalla

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2 arachidonic acid + 2 O2 Strongylocentrotus purpuratus
-
 8-hydroxy,9-oxo-eicosa-5Z,11Z,14Z-trienoic acid + 9-oxo-[8,12-cis]-prosta-5Z,10,14Z-trienoic acid + H2O
-
 ?
2 arachidonic acid + 2 O2 Asteroidea
-
 8-hydroxy,9-oxo-eicosa-5Z,11Z,14Z-trienoic acid + 9-oxo-[8,12-cis]-prosta-5Z,10,14Z-trienoic acid + H2O
-
 ?
2 arachidonic acid + 2 O2 Callista chione
-
 8-hydroxy,9-oxo-eicosa-5Z,11Z,14Z-trienoic acid + 9-oxo-[8,12-cis]-prosta-5Z,10,14Z-trienoic acid + H2O
-
 ?
2 arachidonic acid + 2 O2 Plexaura homomalla
-
 8-hydroxy,9-oxo-eicosa-5Z,11Z,14Z-trienoic acid + 9-oxo-[8,12-cis]-prosta-5Z,10,14Z-trienoic acid + H2O
-
 ?

Organism

Organism UniProt Comment Textmining
Asteroidea
-
 starfish
-
Callista chione
-
 clam, synonym Concha fina
-
Plexaura homomalla
-
 sea whip coral
-
Strongylocentrotus purpuratus
-
 sea urchin
-

Posttranslational Modification

Posttranslational Modification Comment Organism
additional information different values obtained from cDNA and SDS-PAGE imply a post-transitional modification Plexaura homomalla

Purification (Commentary)

Purification (Comment) Organism
-
 Plexaura homomalla

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
352
-
-
 Plexaura homomalla

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2 arachidonic acid + 2 O2
-
 Strongylocentrotus purpuratus 8-hydroxy,9-oxo-eicosa-5Z,11Z,14Z-trienoic acid + 9-oxo-[8,12-cis]-prosta-5Z,10,14Z-trienoic acid + H2O
-
 ?
2 arachidonic acid + 2 O2
-
 Asteroidea 8-hydroxy,9-oxo-eicosa-5Z,11Z,14Z-trienoic acid + 9-oxo-[8,12-cis]-prosta-5Z,10,14Z-trienoic acid + H2O
-
 ?
2 arachidonic acid + 2 O2
-
 Callista chione 8-hydroxy,9-oxo-eicosa-5Z,11Z,14Z-trienoic acid + 9-oxo-[8,12-cis]-prosta-5Z,10,14Z-trienoic acid + H2O
-
 ?
2 arachidonic acid + 2 O2
-
 Plexaura homomalla 8-hydroxy,9-oxo-eicosa-5Z,11Z,14Z-trienoic acid + 9-oxo-[8,12-cis]-prosta-5Z,10,14Z-trienoic acid + H2O
-
 ?
arachidonic acid + O2
-
 Strongylocentrotus purpuratus (8R)-8-hydroperoxy-5,9,11,14-eicosatetraenoic acid
-
 ?
arachidonic acid + O2
-
 Asteroidea (8R)-8-hydroperoxy-5,9,11,14-eicosatetraenoic acid
-
 ?
arachidonic acid + O2
-
 Callista chione (8R)-8-hydroperoxy-5,9,11,14-eicosatetraenoic acid
-
 ?
arachidonic acid + O2
-
 Plexaura homomalla (8R)-8-hydroperoxy-5,9,11,14-eicosatetraenoic acid
-
 ?

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
19.3
-
 arachidonic acid purified enzyme Plexaura homomalla
66.7
-
 arachidonic acid
-
 Plexaura homomalla