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Literature summary for 1.13.11.40 extracted from

  • Jisaka, M.; Iwanaga, C.; Takahashi, N.; Goto, T.; Kawada, T.; Yamamoto, T.; Ikeda, I.; Nishimura, K.; Nagaya, T.; Fushiki, T.; Yokota, K.
    Double dioxygenation by mouse 8S-lipoxygenase: specific formation of a potent peroxisome proliferator-activated receptor alpha agonist (2005), Biochem. Biophys. Res. Commun., 338, 136-143.
    View publication on PubMed
Search for more literature for 1.13.11.40

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli strain BL21 (DE3) Mus musculus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0012
-
 arachidonic acid in 50 mM sodium phosphate buffer, pH 7.4, at 25°C Mus musculus
0.0021
-
 (8R)-8-hydroperoxy-5,9,11,14-eicosatetraenoic acid in 50 mM sodium phosphate buffer, pH 7.4, at 25°C Mus musculus
0.0057
-
 (8S)-hydroxyeicosatetraenoic acid in 50 mM sodium phosphate buffer, pH 7.4, at 25°C Mus musculus
0.015
-
 (15S)-hydroxyeicosatetraenoic acid in 50 mM sodium phosphate buffer, pH 7.4, at 25°C Mus musculus
0.039
-
 (15S)-hydroperoxyeicosatetraenoic acid in 50 mM sodium phosphate buffer, pH 7.4, at 25°C Mus musculus

Localization

Localization Comment Organism GeneOntology No. Textmining

Organism

Organism UniProt Comment Textmining
Mus musculus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni-NTA-agarose column chromatography Mus musculus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(15S)-hydroperoxyeicosatetraenoic acid + O2
-
 Mus musculus ?
-
 ?
(15S)-hydroxyeicosatetraenoic acid + O2
-
 Mus musculus ?
-
 ?
(8R)-8-hydroperoxy-5,9,11,14-eicosatetraenoic acid + O2
-
 Mus musculus (8S,15S)-dihydroperoxy-5Z,9E,11Z,13E-eicosatetraenoic acid
-
 ?
(8S)-hydroxyeicosatetraenoic acid + O2
-
 Mus musculus ?
-
 ?
arachidonic acid + O2
-
 Mus musculus (8R)-8-hydroperoxy-5,9,11,14-eicosatetraenoic acid
-
 ?

Synonyms

Synonyms Comment Organism
8-LOX
-
 Mus musculus
8S-lipoxygenase
-
 Mus musculus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1
-
 (15S)-hydroxyeicosatetraenoic acid in 50 mM sodium phosphate buffer, pH 7.4, at 25°C Mus musculus
1.5
-
 (15S)-hydroperoxyeicosatetraenoic acid in 50 mM sodium phosphate buffer, pH 7.4, at 25°C Mus musculus
3.2
-
 arachidonic acid in 50 mM sodium phosphate buffer, pH 7.4, at 25°C Mus musculus
3.5
-
 (8S)-hydroxyeicosatetraenoic acid in 50 mM sodium phosphate buffer, pH 7.4, at 25°C Mus musculus
7
-
 (8R)-8-hydroperoxy-5,9,11,14-eicosatetraenoic acid in 50 mM sodium phosphate buffer, pH 7.4, at 25°C Mus musculus