Literature summary for 1.13.11.48 extracted from

Steiner, R.A.; Frerichs-Deeken, U.; Fetzner, S.
Crystallization and preliminary X-ray analysis of 1H-3-hydroxy-4-oxoquinaldine 2,4-dioxygenase from Arthrobacter nitroguajacolicus Rue61a: a cofactor-devoid dioxygenase of the alpha/beta-hydrolase-fold superfamily (2007), Acta Crystallogr. Sect. F, 63, 382-385.

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Cloned(Commentary)

Cloned (Comment)	Organism
overexpression of wild-type and mutant N-terminally His6-tagged HODs in Escherichia coli strain M15	Paenarthrobacter nitroguajacolicus

Crystallization (Commentary)

Crystallization (Comment)	Organism
N-terminally His6-tagged HOD is crystallized by the hanging-drop vapour-diffusion method using sodium/potassium tartrate as a precipitant and CuCl2 as an additive. The structure is solved by the single anomalous dispersion technique using data collected to 3.5 A resolution at the Cu absorption peak wavelength. The crystals belong to the primitive tetragonal space group P43212, with unit-cell parameters a = b = 153.788, c = 120.872 A	Paenarthrobacter nitroguajacolicus
purified recombinant wild-type and mutants C69S and C69S/H251A N-terminally His6-tagged HOD, hanging drop vapour diffusion method, 50 mg/ml protein in 20 mM Tris-HCl pH 7.5, 100 mM NaCl, 2 mM EDTA, 1 mM DTT, is mixed with 1.65 M sodium/potassium tartrate, 0.1 M HEPES, pH 7.0, and 30 mM CuCl2, method optimization, X-ray diffraction structure determination and analysis at 3.5 A resolution, single anomalous dispersion technique	Paenarthrobacter nitroguajacolicus

Crystallization (Comment)

Organism

N-terminally His6-tagged HOD is crystallized by the hanging-drop vapour-diffusion method using sodium/potassium tartrate as a precipitant and CuCl2 as an additive. The structure is solved by the single anomalous dispersion technique using data collected to 3.5 A resolution at the Cu absorption peak wavelength. The crystals belong to the primitive tetragonal space group P43212, with unit-cell parameters a = b = 153.788, c = 120.872 A

Paenarthrobacter nitroguajacolicus

purified recombinant wild-type and mutants C69S and C69S/H251A N-terminally His6-tagged HOD, hanging drop vapour diffusion method, 50 mg/ml protein in 20 mM Tris-HCl pH 7.5, 100 mM NaCl, 2 mM EDTA, 1 mM DTT, is mixed with 1.65 M sodium/potassium tartrate, 0.1 M HEPES, pH 7.0, and 30 mM CuCl2, method optimization, X-ray diffraction structure determination and analysis at 3.5 A resolution, single anomalous dispersion technique

Paenarthrobacter nitroguajacolicus

Protein Variants

Protein Variants	Comment	Organism
C69S	site-directed mutagenesis, the mutant has catalytic properties that are identical to those of wild-type HOD	Paenarthrobacter nitroguajacolicus
C69S/H251A	site-directed mutagenesis, the mutant is catalytically inactive owing to the Ala substitution of the essential residue His251	Paenarthrobacter nitroguajacolicus

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.
1H-3-Hydroxy-4-oxoquinaldine + O2	Paenarthrobacter nitroguajacolicus	-	N-Acetylanthranilate + CO	-	?
1H-3-Hydroxy-4-oxoquinaldine + O2	Paenarthrobacter nitroguajacolicus Rü61a	-	N-Acetylanthranilate + CO	-	?
1H-3-Hydroxy-4-oxoquinaldine + O2	Paenarthrobacter nitroguajacolicus Rue61a	-	N-Acetylanthranilate + CO	-	?

Organism

Organism	UniProt	Comment	Textmining
Paenarthrobacter nitroguajacolicus	-	-	-
Paenarthrobacter nitroguajacolicus Rue61a	-	-	-
Paenarthrobacter nitroguajacolicus Rü61a	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
recombinant wild-type and mutant N-terminally His6-tagged HODs from Escherichia coli strain M15 by nickel affinity and anion exchange chromatography	Paenarthrobacter nitroguajacolicus

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.
1H-3-Hydroxy-4-oxoquinaldine + O2	-	Paenarthrobacter nitroguajacolicus	N-Acetylanthranilate + CO	-	?
1H-3-Hydroxy-4-oxoquinaldine + O2	-	Paenarthrobacter nitroguajacolicus Rü61a	N-Acetylanthranilate + CO	-	?
1H-3-Hydroxy-4-oxoquinaldine + O2	-	Paenarthrobacter nitroguajacolicus Rue61a	N-Acetylanthranilate + CO	-	?

Subunits

Subunits	Comment	Organism
More	three-dimensional structure analysis, overview	Paenarthrobacter nitroguajacolicus

Synonyms

Synonyms	Comment	Organism
1H-3-Hydroxy-4-oxoquinaldine 2,4-dioxygenase	-	Paenarthrobacter nitroguajacolicus
HOD	-	Paenarthrobacter nitroguajacolicus
More	HOD belongs to the alpha/beta-hydrolase-fold superfamily of enzymes	Paenarthrobacter nitroguajacolicus