Literature summary for 1.13.11.49 extracted from

Freire, D.M.; Rivas, M.G.; Dias, A.M.; Lopes, A.T.; Costa, C.; Santos-Silva, T.; Van Doorslaer, S.; Gonzalez, P.J.
The homopentameric chlorite dismutase from Magnetospirillum sp (2015), J. Inorg. Biochem., 151, 1-9 .

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Crystallization (Commentary)

Crystallization (Comment)	Organism
enzyme is a homopentamer, with presence of one b-type heme per monomer	Magnetospirillum sp.

Inhibitors

Inhibitors	Comment	Organism	Structure
chloride	weak mixed inhibitor that modifies the EPR signal of as-prepared samples	Magnetospirillum sp.

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.59	-	Chlorite	pH 6.5, 10°C	Magnetospirillum sp.
1.7	-	Chlorite	pH 6.0, 10°C	Magnetospirillum sp.
1.7	-	Chlorite	pH 5.5, 10°C	Magnetospirillum sp.

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
140000	-	gel filtration	Magnetospirillum sp.

Organism

Organism	UniProt	Comment	Textmining
Magnetospirillum sp.	A0A0M3KL46	-	-

Purification (Commentary)

Purification (Comment)	Organism
the EPR spectroscopic signature of the as-purified Cld samples is affected by the buffer composition. Potassium phosphate buffer is the only buffer that affects neither the spectral nor the kinetic properties of Cld	Magnetospirillum sp.

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
chlorite	-	Magnetospirillum sp.	chloride + O2	-	?

Subunits

Subunits	Comment	Organism
pentamer	5 * 27800, MALDI-TOF	Magnetospirillum sp.

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
10	-	temperature below 10°C is required to avoid enzyme inactivation due to cofactor bleaching during turnover, and to achieve full substrate consumption	Magnetospirillum sp.

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
6	-	-	Magnetospirillum sp.

Cofactor

Cofactor	Comment	Organism	Structure
heme	presence of one b-type heme per monomer	Magnetospirillum sp.

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Release 2023.1 (January 2023)