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Literature summary for 1.13.11.5 extracted from

  • Fernandez-Canon, J.M.; Penalva, M.A.
    Spectrophotometric determination of homogentisate using Aspergillus nidulans homogentisate dioxygenase (1997), Anal. Biochem., 245, 218-221.
    View publication on PubMed
Search for more literature for 1.13.11.5

Activating Compound

Activating Compound Comment Organism Structure
ascorbate dependent on the presence of ascorbate, presumably to maintain iron in the reduced form Aspergillus nidulans
additional information enzymes of phenylalanine catabolism including HGO are strongly induced by the presence of phenylalanine or phenylacetate Aspergillus nidulans

Application

Application Comment Organism
diagnostics crude enzyme preparations can be used for a spectrophotometric method for routine, sensitive determination of homogentisate in human urine Aspergillus nidulans

Protein Variants

Protein Variants Comment Organism
additional information strain carrying an homogentisate dioxygenase gene disruption with full genotype biA1, methG1, deltahmgA without enzyme activity Aspergillus nidulans

General Stability

General Stability Organism
rather stable enzyme, can be repeatedly thawed and frozen without significant loss of activity Aspergillus nidulans

Inhibitors

Inhibitors Comment Organism Structure
3,4-Dihydroxyphenylacetate 1 mM, 48% inhibition, substrate analogue Aspergillus nidulans
p-hydroxyphenylpyruvate 1 mM, 68% inhibition, substrate analogue Aspergillus nidulans

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.009
-
 homogentisate
-
 Aspergillus nidulans

Metals/Ions

Metals/Ions Comment Organism Structure
Fe2+ dependent on the presence of Fe2+ Aspergillus nidulans

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
homogentisate + O2 Aspergillus nidulans one of the enzymes mediating phenylalanine catabolism 4-maleylacetoacetate
-
 ?

Organism

Organism UniProt Comment Textmining
Aspergillus nidulans
-
 filamentous ascomycete
-
Aspergillus nidulans
-
 strains biA1 and veA1
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.75 1 value in crude lysates Aspergillus nidulans

Storage Stability

Storage Stability Organism
-20°C, 50 mM potassium phosphate buffer, pH 7.0, crude extracts, 5 months, 25% loss of activity Aspergillus nidulans

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
homogentisate + O2 highly specific for homogentisate Aspergillus nidulans 4-maleylacetoacetate
-
 ?
homogentisate + O2 one of the enzymes mediating phenylalanine catabolism Aspergillus nidulans 4-maleylacetoacetate
-
 ?
additional information not as substrates: phenylacetate, 2-hydroxyphenylacetate, 3-hydroxyphenylacetate, 4-hydroxyphenylacetate, phenylalanine, tyrosine, phenylpyruvate, gentisate Aspergillus nidulans ?
-
 ?

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.5 7
-
 Aspergillus nidulans