Activating Compound | Comment | Organism | Structure |
---|---|---|---|
ascorbic acid | tryptophan 2,3-dioxygenase: maximal activity at 10 mM ascorbate | Rattus norvegicus | |
hyperbaric oxygen | - |
Rattus norvegicus | |
hyperbaric oxygen | - |
Meriones unguiculatus | |
O2- | - |
Rattus norvegicus | |
O2- | - |
Meriones unguiculatus |
Application | Comment | Organism |
---|---|---|
medicine | rate-limiting enzyme in kynurenine pathway | Rattus norvegicus |
medicine | rate-limiting enzyme in kynurenine pathway | Meriones unguiculatus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
2.8 | - |
O2 | indoleamine 2,3-dioxygenase | Meriones unguiculatus | |
2.8 | - |
oxygen | pH 6.5, 37°C | Rattus norvegicus | |
2.8 | - |
oxygen | pH 6.5, 37°C | Meriones unguiculatus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-tryptophan + O2 | Rattus norvegicus | - |
N-formyl-D-kynurenine | - |
? | |
D-tryptophan + O2 | Meriones unguiculatus | - |
N-formyl-D-kynurenine | - |
? | |
L-Trp + O2 | Rattus norvegicus | indoleamine 2,3-dioxygenase and tryptophan 2,3-dioxygenase catalyze the rate-limiting step in the kynurenine pathway from Trp to quinolinic acid | L-formylkynurenine | - |
? | |
L-Trp + O2 | Meriones unguiculatus | indoleamine 2,3-dioxygenase and tryptophan 2,3-dioxygenase catalyze the rate-limiting step in the kynurenine pathway from Trp to quinolinic acid | L-formylkynurenine | - |
? | |
L-tryptophan + O2 | Rattus norvegicus | no activity with oxygen concentrations below 0.1 mM, maximum activity at 1.15 mM oxygen | N-formyl-L-kynurenine | - |
? | |
L-tryptophan + O2 | Meriones unguiculatus | no activity with oxygen concentrations below 0.1 mM, maximum activity at 1.15 mM oxygen | N-formyl-L-kynurenine | - |
? | |
L-tryptophan + O2- | Rattus norvegicus | - |
N-formyl-L-kynurenine | - |
? | |
L-tryptophan + O2- | Meriones unguiculatus | - |
N-formyl-L-kynurenine | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Meriones unguiculatus | - |
- |
- |
Meriones unguiculatus | - |
indoleamine 2,3-dioxygenase | - |
Rattus norvegicus | - |
Sprague Dawley | - |
Rattus norvegicus | - |
indoleamine 2,3-dioxygenase from small intestine | - |
Rattus norvegicus | - |
tryptophan 2,3-dioxygenase from liver | - |
Purification (Comment) | Organism |
---|---|
tissues thawed at room temperature, homogenized in 0.14 M potassium chloride plus 20 mM potassium phosphate, pH 7.0 | Rattus norvegicus |
tissues thawed at room temperature, homogenized in 0.14 M potassium chloride plus 20 mM potassium phosphate, pH 7.0 | Meriones unguiculatus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
brain | - |
Rattus norvegicus | - |
brain | - |
Meriones unguiculatus | - |
brain | indoleamine 2,3-dioxygenase | Meriones unguiculatus | - |
liver | tryptophan 2,3-dioxygenase | Rattus norvegicus | - |
lung | - |
Meriones unguiculatus | - |
lung | indoleamine 2,3-dioxygenase | Meriones unguiculatus | - |
small intestine | - |
Rattus norvegicus | - |
small intestine | indoleamine 2,3-dioxygenase | Rattus norvegicus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.011 | - |
brain, in air, 50 mM potassium phosphate buffer, pH 6.5, 0.025 mM methylene blue, 20 mM ascorbate, 0.05 mM catalase, 0.4 mM L-tryptophan, 37°C | Meriones unguiculatus |
0.014 | - |
brain, in 1.2 atm oxygen, 50 mM potassium phosphate buffer, pH 6.5, 0.025 mM methylene blue, 20 mM ascorbate, 0.05 mM catalase, 0.4 mM L-tryptophan, 37°C | Meriones unguiculatus |
0.07 | - |
brain, in 5.2 atm oxygen, 50 mM potassium phosphate buffer, pH 6.5, 0.025 mM methylene blue, 20 mM ascorbate, 0.05 mM catalase, 0.4 mM L-tryptophan, 37°C | Meriones unguiculatus |
0.145 | - |
small intestine, in 5.2 atm oxygen, 50 mM potassium phosphate buffer, pH 6.5, 0.025 mM methylene blue, 20 mM ascorbate, 0.05 mM catalase, 0.4 mM L-tryptophan, 37°C | Rattus norvegicus |
0.198 | - |
lung, in air, 50 mM potassium phosphate buffer, pH 6.5, 0.025 mM methylene blue, 20 mM ascorbate, 0.05 mM catalase, 0.4 mM L-tryptophan, 37°C | Meriones unguiculatus |
0.24 | - |
small intestine, in 1.2 atm oxygen, 50 mM potassium phosphate buffer, pH 6.5, 0.025 mM methylene blue, 20 mM ascorbate, 0.05 mM catalase, 0.4 mM L-tryptophan, 37°C | Rattus norvegicus |
0.38 | - |
small intestine, in air, 50 mM potassium phosphate buffer, pH 6.5, 0.025 mM methylene blue, 20 mM ascorbate, 0.05 mM catalase, 0.4 mM L-tryptophan, 37°C | Rattus norvegicus |
0.873 | - |
lung, in 5.2 atm oxygen, 50 mM potassium phosphate buffer, pH 6.5, 0.025 mM methylene blue, 20 mM ascorbate, 0.05 mM catalase, 0.4 mM L-tryptophan, 37°C | Meriones unguiculatus |
0.905 | - |
lung, in 1.2 atm oxygen, 50 mM potassium phosphate buffer, pH 6.5, 0.025 mM methylene blue, 20 mM ascorbate, 0.05 mM catalase, 0.4 mM L-tryptophan, 37°C | Meriones unguiculatus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-tryptophan + O2 | - |
Rattus norvegicus | N-formyl-D-kynurenine | - |
? | |
D-tryptophan + O2 | - |
Meriones unguiculatus | N-formyl-D-kynurenine | - |
? | |
L-Trp + O2 | tryptophan 2,3-dioxygenase activity is appreciable even at 0.03 mM oxygen and rises steeply to a maximum at 0.04 mM | Rattus norvegicus | L-formylkynurenine | - |
? | |
L-Trp + O2 | indoleamine 2,3-dioxygenase has almost no detectable activity at or below 0.1 mM oxygen and maximum activity at about 1.15 mM | Meriones unguiculatus | L-formylkynurenine | - |
? | |
L-Trp + O2 | indoleamine 2,3-dioxygenase and tryptophan 2,3-dioxygenase catalyze the rate-limiting step in the kynurenine pathway from Trp to quinolinic acid | Rattus norvegicus | L-formylkynurenine | - |
? | |
L-Trp + O2 | indoleamine 2,3-dioxygenase and tryptophan 2,3-dioxygenase catalyze the rate-limiting step in the kynurenine pathway from Trp to quinolinic acid | Meriones unguiculatus | L-formylkynurenine | - |
? | |
L-tryptophan + O2 | no activity with oxygen concentrations below 0.1 mM, maximum activity at 1.15 mM oxygen | Rattus norvegicus | N-formyl-L-kynurenine | - |
? | |
L-tryptophan + O2 | no activity with oxygen concentrations below 0.1 mM, maximum activity at 1.15 mM oxygen | Meriones unguiculatus | N-formyl-L-kynurenine | - |
? | |
L-tryptophan + O2- | - |
Rattus norvegicus | N-formyl-L-kynurenine | - |
? | |
L-tryptophan + O2- | - |
Meriones unguiculatus | N-formyl-L-kynurenine | - |
? |